A Preliminary Study On The Relation Between Stroke And Trimethylamine N-oxide And The Effect Of Tan-Huo-Fang On Trimethylamine N-oxide

ObjectiveTo explore the relation between atheroselerotic cerebrovascular disease and Trimethylamine N-oxide,and then analyze the impact of traditional Chinese medicine on Trimethylamine N-oxide.MethodsLiquid chromatography mass spectrometry(HPLC-MS/MS)was used to analyze the level of plasma trimethylamine oxide in healthy volunteers and patients.The patients were divided into two groups: Chinese medicine treatment group and without Chinese medicine treatment group.Before treatment,plasma trimethylamine oxide in people in the study group and healthy control group were collected,after 7 days of treatment,Repeat this process.All plasma samples were frozen at-80℃ refrigerator(labeled group).Liquid chromatography mass spectrometry(HPLC-MS/MS)was used to analyze the level of plasma trimethylamine oxide in blood samples.Allchromatographic data were calculated in accordance with the peak area calculated by the cooperative unit chemical workstation.The level of TMAO before treatment was compared with that of healthy control group.The level of plasma trimethylamine oxide in patients were compared before and after treatment.The level of plasma trimethylamine oxide in Chinese medicine treatment group after treatment was compared with that of without Chinese medicine treatment group.ResultsThe level of plasma trimethylamine oxide before treatment in the treatment group was significantly different from that in the healthy control group.Plasma trimethylamine oxide levels in healthy controls were lower than those in the treatment group before treatment.There was a difference in plasma trimethylamine oxide levels before and after treatment in the treatment group,and plasma trimethylamine oxide levels decreased after treatment.For low density lipoprotein,total cholesterol,trimethylamine oxide,fire score changes,there was a statistically significant difference between the phlegm fire group and the non phlegm fire group,and the change in the phlegm fire group was greater than that in the non phlegm fire group.There was no linear correlation between body mass index,triglyceride,high-density lipoprotein,low density lipoprotein,total cholesterol,blood glucose and trimethylamine oxide.The age and the degree of phlegm heat syndrome were positively correlated with trimethylamine oxide.ConclusionHigh level plasma trimethylamine oxide is a risk factor for atherosclerotic ischemic cerebrovascular disease.The phlegm fire recipe has the function of assisting western medicine,reducing plasma trimethylamine oxide level,LDL,TCH,and burning syndrome score.There was a positive correlation between age and fever score and trimethylamine oxide.