Effect Of Dimethylaminoethano And Compound Amino Acid On Periostin And Dermatopontin Expression In The Skin Of D-galactose Induced Aging Model In Rats

Objective: To study the effect of dimethylaminoethano(DMAE)and compound amino acid inject(AA)by mesotherapy on Periostin and Dermatopontin expression levels of D-galactose-induced chemical skin aging rat.Method: By using 80 randomly selected wistar rats,randomly divided into normal control group,the aging control group and the experimental group.On the 18 th day after induction of D-gal,the experimental group were treated with intra-dermal microinjection of 0.2%DMAE+AA,0.1%DMAE+AA,0.2%DMAE,0.1%DMAE,AA and NS on bilateral buttocks respectively,injecting for once a week,consecutively for four weeks.Treatment after 42 days,observe the histological changes in the dermal thickness,hydroxyproline content,the expression of collagen I and collagen III m RNA expression levels,and the expression of Periostin and Dermatopontin level in every individual group.Results: Mesotherapy with intradermal co-injection of 0.1%DMAE+AA and 0.2%DMAE+AA has significantly increased the dermal thickness,Hydroxyproline content,expression level of collagen I and collagen III m RNA,and Periostin expression levels increased significantly,but did not affect the expression of Dermatopontin.Conclustion: By local co-injection of DMAE and AA compound directly under aged rats can effectively reform the aging of skin tissue structure and denatured collagen?Meanwhile it can improve collagen synthesis in aging skin and significantly increases dermal thickness and collagen content of the skin,and thus prevent skin aging.