The Role And Mechanism Of Mitogen-induced Gene 6 In Migration And Proliferation Of Gastric Cancer

Objective:To detect expression of mitogen-inducible gene-6 in normal gastric mucosa epithelial cells and gastric cancer cell lines and human gastric cancer tissues and adjacent non-cancerous tissues and different human gastric cancer cell lines,search for the molecular mechanism of MIG-6 on gastric cancer cell proliferation and migration.To provide new ideas and theoretical basis for molecular targeted therapy of gastric cancer.Methods:Immunohistochemistry and qRT-PCR were applied to detect MIG-6 mRNA and protein expression in gastric cancer tissues and adjacent non-cancerous tissues;Using Western blot to detect the expression of MIG-6 in normal gastric mucosa epithelial cells and gastric cancer cell lines.RNAi inhibit MIG-6 expression in gastric cancer cell,using Western blot to detect the expression of EMT related genes such as E-cadherin,N-cadherin,snail and vimentin,as well as through CCK-8 cell proliferation experiment,plate clone formation test,transwell migration experiment to observe the effects on gastric cancer cell proliferation and migration ability after inhibiting MIG-6expression.We constructed the MIG-6 overexpression plasmid and transfected it into BGC-823 gastric cancer cell line to observe the expression of EMT-related gene and the effect of overexpression on cell proliferation and migration.Results:The results of immunohistochemistry showed that MIG-6 expression in gastric cancer tissues was higher than that in adjacent tissues.It was obtained by qRT-PCR and ONCOMINE database analysis that the expression of MIG-6 in gastric cancer tissue was higher than the corresponding adjacent non-cancerous tissues in mRNAlevel.Inhibition of MIG-6 by siRNA in human gastric cancer SGC-7901 cells led to reduced the ability of cell migration and the number of cell clone formation,inhibition of EMT,with increased E-cadherin and decreased snail,N-cadherin,vimentin level.Overexpression of MIG-6 by plasmid in human gastric cancer BGC-823 cells led to increased the ability of cell migration and the number of cell clone formation,promotion of EMT,with decreased E-cadherin and increased snail,N-cadherin,vimentin level.Conclusions:Mitogen-inducible gene 6 has a certain effect on the carcinogenesis and progression of gastric cancer and may promote the migration of gastric cancer cells by inducing EMT.MIG-6 will likely become the new targets of gastric cancer molecular diagnosis and provide new way for the clinical treatment of gastric cancer.