Effects Of Xingnaojing Injection Combined With Butylphthalide(NBP) On The Expression Of Apoptosis And Neuroprotective Effects After Cerebral Ischemia Reperfusion

Objective To observe the effects of Xingnaojing injection,butylphthalide(NBP)and combined with them on the expression of apoptosis,Bcl-2 and Bax in rats after cerebral ischemia reperfusion.To investigate whether there are protective effects on brain,and to explore its possible mechanism.Methods Sixty male Wistar rats by modified suture method for cerebral ischemia reperfusion(MCAO)were randomly assigned into four groups : model group,Xingnaojing group,butylphthalide(NBP)group,combined with NBP and xingnaojing(combination)group.Each group was divided into three subgroups:6h,24 h and 72 h.The apoptosis of nerve cells was detected by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling(TUNEL),and the expression of Bcl-2 and Bax were detected by immunohistochemistry.Results In the model group,a large number of apoptotic cells were observed in the lesion area.In NBP group and Xingnaojing group,the number of apoptosis were significantly reduced,the combination group at the least level(P <0.05).In NBP group and combination group,the expression of bcl-2 was significantly lower than that in the model group.the combination group at the highest level,at each time point(P < 0.05).The expression of bax was to the contrary(P < 0.05).Conclusion 1.The use of xingnaojing,BNP and combination of the two have neuroprotective effects through the inhibition of nerve cell apoptosis,in which the combination group do the best.2.NBP can relieve the brain damage by up-regulating the expression of Bcl-2 and down-regulating the expression of Bax.3.Xingnaojing injection itself cannot influence the expression of bcl-2 and Bax.but it can enhance the effect of BNP to the expression of bcl-2 and Bax.It can indirectly alleviate cerebral ischemia reperfusion injury.