3D Coaxial Print Bone Tissue Engineering Scaffold Molding Process And Experimental Research

For caused by mycobacterium tuberculosis invades the bone or joint bone tuberculosis lesions,in order to improve the drug utilization of lesion area and achieve the aims of efficient treatment of bone tuberculosis and rapid repair the bone defects,combining the rapid prototyping technique(RP),tissue engineering technology(TE)and biological drug,built on the basis of bone tissue engineering scaffolds of implantable drug delivery system.It provides a new way of thinking and an effective method for the treatment of bone tuberculosis.This thesis is based on material selection and coaxial structure building of coaxial composite bone tissue engineering scaffolds as the key starting point,build the coaxial 3DP test platform and design the coaxial nozzle extrusion device,study on the optimal ratio of the silk fibroin(SF)and poly vinyl alcohol(PVA)and the preparation of the SF/PVA composite hydrogel,and hydroxyapatite(HA)mixed preparation of SF/PVA/HA composite material as the inner core toughening material of coaxial scaffold.at the same time,using PVA /HA composite material as shell material.The paper adopt mechanical coaxial extrusion rapid prototyping technology for preparing coaxial scaffold,analysis of the technical parameters of the coaxial scaffold printing process,material mixing ratio,proportion of coaxial nozzle,scanning speed,the extrusion speed and other factors influence on the forming accuracy of coaxial scaffold,optimization of key process parameters values,preparation of coaxial composite scaffolds bone tissue engineering scaffold which has the merits of variety composite materials,good macro morphology,uniform pore structure and high degree of coaxial wire.The cytotoxicity experiments of the osteogenic potential and biocompatibility verify the feasibility and validity of the results.At the same time,it can verify the degradation rate of replantation of stent and osteogenesis performance by forming scaffold degradation in vitro and animal experiments,provide experimental basis for the research and application of implantable bone tissue engineering scaffold based drug delivery system.