Transcriptome Analysis Of Hepatoma HepG2 Cells Induced By Peptide 9R-P201 And Study On The Regulation And Function Of Long Noncoding RNA RP11-224O19.2

Previous study demonstrated that 9R-P201 could inhibit the survival,proliferation,migration and induce apoptosis of hepatocellular carcinoma (HCC) HepG2 cells. Long non-coding RNAs (lncRNAs) are a class of noncoding RNA with length more than 200 nucleotides, studies have confirmed that lncRNAs participate the tumorigenesis and progression of tumors via regulating gene expression at epigenetic, transcriptional and posttranscriptional level. However, the function and mechanism of 9R-P201 on mRNA and IncRNA expression profiling of HepG2 cells is still unclear.In this study, we performed the transcriptome sequencing analysis of the HepG2 cells treated with 9R-P201, and identified 1557 mRNAs and 881 IncRNAs with significant differential expression. The comparative analysis of structural characteristics revealed that mRNAs differed greatly from lncRNAs in gene length, transcript length, ORF length, exon number, isoform number. Gene ontology (GO) and KEGG pathway enrichment analysis demonstrated that differentially expressed mRNAs and lncRNAs were significantly involved in cancer-related biological processes and signaling pathways. Based on the transcription factor (TF) binding site and protein interaction analysis, we screened out 33 transcription factors, 333 lncRNAs and 94 target genes with high interaction enrichment via betweenness centrality (BC), and then constructed the regulatory network. We found that there was 634 interaction relationships between the differentially expressed mRNAs, and then constructed the mutual interaction network. The qRT-PCR result showed that the expression of the differentially expressed mRNAs and lncRNAs was consistent with the RNA-seq results.Next, lncRNA RP11-224O19.2 with has not been in HCC. was selected for function research.The analysis showed RP11-224019.2 was increased in HCC tissues and associated with tumor stage. Subsequently, RP11-224O19.2 depiction significantly inhibited the proliferation, colony formation, migration, invasion and promoted apoptosis of HepG2 cells.Finally, we found that TGFB2, an oncogene overlapped with RP11-224O19.2, was positively correlated with RP11-224019.2. Moreover, the expression of TGFB2 was reduced by 88% upon RP11-224O19.2 depletion, suggesting that TGFB2 may be the potential target gene of RP11-224O19.2.