Study On The Mechanism Of Genistein Regulating Colon Tumor By Inhibiting Lipid Accumulation

Colon cancer is the third most malignant tumor in the world. Obesity is one of the risk factors of colon cancer among many influencing factors. The present study found that the synthesis of fat and secretion of some factors associated with the occurrence and develop-ment of colon cancer. In this paper, the mechanism of genistein in preventing colonic neo-plasms was discussed in order to investigate the molecular mechanism of the effect of genistein on the occurrence and development of colorectal cancer.(1) The effects of genistein on the tumorigenesis rate of colonic tumors were detected by in vivo experiments. The results showed that the addition rate of genistein was negatively correlated with the tumorigenicity rate: high tumor rate (450mg / kg) Dose rate(150mg / kg)<Low dose tumor formation rate (50mg / kg)<Model tumor formation rate. (2)The results of serum test showed that obesity increased TG, TC, LDL-C, HDL-C and lipase activity in mice. In addition, genistein significantly inhibited the increase of TG, TC and LDL-C in the serum induced by high-fat diet, but the effect of genistein on TG, TC and LDL-C was not significant. TCL and LDL-C in serum of AOM / DSS-induced colonic neoplasms mice, and increase the content of HDL-C and lipase activity in serum. . (3) The gene expression level in mouse colonic tissue was detected by RT-PCR. The results showed that the uptake of genistein could decrease the expression of FAS, ACC1 and b-FABP mRNA and increase the mRNA expression of MTTP and SCD1 in the colon. (4) Western Blot was used to detect the expression of protein in mouse colon. The results showed that genistein can decrease the expression of perilipin, ADRP and TIP-47 protein in the colonic tissue of colonic tumors and high-fat mice, and decrease the accumulation of fat droplets in colon tissue cells. It can also inhibit the growth of tumor by inhibiting the activity of PPAR-? protein in colonic tumor and high-fat mouse colonic tissue, so as to up-regulate SIRT and FOXO3.The results of this study show that genistein intake can reduce the accumulation of mouse colon tissue fat, which play a role in the prevention of colon cancer.