| Rheumatoid Arthritis(RA)is a common chronic autoimmune disease,which ultimatelyleads to the destruction of the joint architecture.The goals of management of patients with RA are to contral pain and swelling,delay disease progression,minimize disability,and improve quality of life.For pain control and swelling,treatment includes analgesics such as acetaminophen and opioids.NSAIDs,and intra-articular therapies such as glucocortucoids.In assition,diseasemodifying anti-rheumatic drugs(DMARDs)are used tomodifying the clinical and radiological course of RA.The precise pathogenic mechanisms leading to the development of RA are proinflammatory cytokines,such as tumor necrosis factor-a(TNF-α),interleukin(IL)-1 and IL-6 play roles in the induction of RA.Preliminary studies of our study group showed that the ethanol extract of Smilax riparia A.DC have a significant inhibitory effect.Anti-inflammatory activity was determined in secondary inflammation rats in a model of Freund’s complete adjuvant arthritis as well as acute inflammation rats in a model of Carrageenan paw swelling.The ethanol extract of Smilax riparia A.DC caused a significant inhibition of TNF-a,IL-1β and IL-6.This article study on the anti-rheumatoid arthritis effection and chemical components of Smilax riparia A.DC base on proinflammatory cytokine targets,15 compounds are isolated from the ethyl acetate soluble fractions of ethanolic extract of Smilax riparia A.DC by modern isolation and identification of technology,which include 11 phenylpropanoid glycosides,(3,6-O-diferuloyl)-β-D-fructofuranosyl-(2→1)-(4’-O-acetyl)-a-D-glucopyranoside(smilaside O N3),(3-O-Z-feruloyl-6-O-feruloyl)-β-D-fructofuranosyl-(2→l)-(2’,6’-O-diacetyl)-α-D-glucopyranoside(smilaside M N4),(3-O-feruloyl-6-O-Z-feruloyl)-β-D-fructofuranosyl-(2→1)-(2’,6’-O-diacetyl)-a-D-glucopyranoside(smilaside N N5),(3,6-O-diferuloyl)-β-D-fructofuranosyl-(2→1)-(2’,6’-O-diacetyl)-a-D-glucopyranoside(N6),(3,6-O-diferuloyl)-β-D-fructofuranosyl-(2→1)-(4’,6’-O-diacetyl)-a-D-glucopyranoside(N7),(3,6-O-diferuloyl)-β-D-fructofuranos yl-(2→1)-(1,6’-O-diacetyl)-a-D-glucopyranoside(smilaside P N8),(3,6-O-diferuloyl)-β-D-fructofuranosyl-(2→1)-(2’-O-acetyl)-a-D-glucopyranoside(N9),(3,6-O-diferuloyl)-β-D-fructofuranosyl-(2→1)-(6’-O-acetyl)-a-D-glucopyranoside(N10),(1,3,6-O-triferuloyl)-β-D-fructofuranosyl-(2→1)-(2’-O-acetyl)-a-D-glucopyranoside(smilaside Q N11),(1,3,6-O-triferuloyl)-β-D-fructofuranosyl-(2→1)-α-D-glucopyranoside(N12),(3,6-O-diferuloyl)-β-D-fructofuranosyl-(2→1)-a-D-glucopyranoside(N14);I terpene,2a-hydroxy oleanolic acid(N2);3 saponins,20(S)ginsenoside-Rhi(N15),neotigogenin-3-O-a-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(N16),dios genin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside(N 17).Among them,five new compounds N3,N4,N5,N8 and N11 were isolated from Smilax riparia A.DC N2,N15and N17 were isolated for the first time from Smilax.Preliminary studies of our study group showed that the chloroform and ethanol extract of Clematis manshurica Rup.have a significant inhibitory effect.Anti-inflammatory activity was determined in secondary inflammation rats in a model of Freund’s complete adjuvant arthritis and in acute inflammation rats in a model of Carrageenan paw swelling.The ethanol extract of Clematis manshurica Rup.caused a significant inhibition of TNF-α,IL-1β and IL-6.This article study on the anti-rheumatoid arthritis effection and chemical components of Clematis manshurica Rup.base on proinflammatory cytokine targets,13 lignans are isolated from the chloroform and ethyl acetate soluble fractions of ethanolic extract of Clematis manshurica Rup by modern isolation and identification of technology,the structures were identified as(+)-epipinoresinol(T1),(+)-pinoresinol(T2),(-)-episyringaresinol(T3),(+)-medioresinol(T4),(+)-pinoresinol-4-0-β-D-glucopyranoside(T5),(+)-syringaresinol-4-0-β-D-glucopyranoside(T6),(+)-epis yringaresinol-4-0-β-D-glucopyranoside(T7),matairesinol(T8),(+)-lyoniresinol(T9),(+)-isolariciresinol(T10),(7R,8R)-4,7,9,9’-tetrahydroxy-3,3’-diethoxy-8-O-4’-neolignan(T11),(7R,8S)-4,7,9,9’-tetrahydroxy-3,3’-diethoxy-8-O-4’-neolignan(T12),(7S,8R)-dihydrodehydroconiferyl alcohol(T13).T1,T3,T4,T7,T8,T9,T11 and T12 were isolated for the first time from Clematis.T1-T13 were isolated for the first time from the air part of Clematis manshurica Rup.This paper lay the foundation for the determination of activity on proinflammatory cytokine targets of Smilax riparia A.DC and Clematis manshurica Rupr. |
