Modification And Performance Of Collagen Membrane

Objective:The use of collagen membrane is becoming more and more widely in regeneration medicine area because of its excellent biocompatibility.But the poor mechanical properties and uncontrollable degradation are still the weakness of collagen.Chemical cross-linking and biomimetic mineralization were used to modify the collagen membrane,and the properties before and after crosslinking,before and after treatment,to look for methods to improve degeneration controllability and mechanical strength,for better performance and clinical applications.Methods:The finished(Heal-All)collagen membrane was used.Frist,collagen membrane were crosslinked,using genipin and carbodiimide,in different concentrations.The crosslinking reaction was carried out at a temperature of 37?for 24 hours.The difference before and after crosslinking were examined.Seconds,simulated body fluid,alternative soaking were used.Plan one:at first,collagen membrane with or without pre-mineralization,was immersed in simulated body fluids for different periods of mineralization,and in the other way,the collagen film was alternately placed in a solution which was rich in calcium and phosphorus ions,collagen membrane was as a template to guide the mineralization.All the treatment was carried out at 37?.The degradation time of different mineralization condition was observed in vitro.Results:(1)The collagen membrane was successful crosslinked by Genipin and EDC/NHS,and the mechanical strength and the anti-degradation ability of the collagen film was improved.(2)At 37?,the optimum crosslinking concentration of Genipin was different.The mechanical strength of the collagen membrane crosslinked by 0.375%and 0.625%Genipin were the best.The anti-degradation ability was stronger after treatment of 0.5%.(3)At 37?,pH = 5.5,24 hours,the optimum crosslinking concentration of EDC/NHS was 50mmol/L,while the properties of crosslinked collagen membrane were best.(4)The alternative mineralization with 6-cycles was more effective on collagen film mineralization,but after this treatment,the membrane structure was incomplete and the membrane pores was disappeared,because of faster degeneration.(5)The method of pre-mineralization + SBF had successfully achieved mineralization.Compared with the group without pre-mineralization,the required mineralization time was shortened.The group of pre-mineralization + SBF mineralization 8 days,had best anti-degradation ability.Conclusion:In this study,a new type of collagen membrane with higher mechanical strength and better resistance to enzyme was successfully prepared by chemical crosslinking method.The collagen membrane was successfully mineralized by the method of biomimetic mineralization.The collagen membrane was better modified by pre-mineralization + SBF.After mineralization,the collagen membrane has better degeneration ability and osteoconductive property.The collagen membrane prepared by alternating mineralization method was weaker in enzymatic hydrolysis.Through the above different ways,the properties of collagen membrane could be adjused to a proper condition for meeting the different clinical needs.