To Split Bushenzhuanggu Effective Parts Of Achyranthes And Study Its Pharmacological Effects

Radix Achyranthis Bidentatae,the dry root of Achyranthes hidentatu BI,also known as hundredfold,Hill amaranth,Jimingniujing Etc.Amaranthaceae hyssop is a perennial herb.Achyranthes have strong bones,liver and kidney.promoting blood circulation,diuretic Tonglin,blood lead(fire)downstream and other effects,clinical application has a iong history.Our previous studies found and confirmed Bushenzhuanggu effective parts of Achyranthes.on the basis of this paper,the effective parts of the extraction process optimization,chemical split and pharmacological effects of the split1 Study on the extraction of effective partsThrough single factor experiment to determine the effects of Achyranthes range of effective parts of the extraction process,application response surface methodology(RSM)obtained optimum conditions Achyranthes effective part of the cream rates are as follows:50%ethanol extraction solvent,extraction time 4h,extracted 3 times,the water feed ratio of 15:1.Response surface analysis to predict the rate of 4.53%cream,extract the effective parts of three batches of experimental verification by.the results show that the actual yield and efficiency of the site is very close to the predicted value.2 Split and determination of the effective part of AchyranthesIn this paper,the effective parts of Achyranthes was split by D941 resin,achyranthes steroidal component and achyranthes saponin,Do not cross validation performed by UPLC-QTOF-MS chromatographic methods combined with PCA and OLC-DA analysis tools.The results showed that:steroidal saponin constituent components and do not cross the split was good.Content was determined using UV spectrophotometry.The measurement results show the total steroid content of 53.3%Achyranthes steroidal components;accounted for 64.8%of total saponin content Achyranthes saponin components.3 Pharmacodynamic studies of Achyranthes split componentsOf the impact of osteoporosis induced rat model of each component study on osteoporosis-related biochemical indicators,found that steroid component can significantly reduce serum ALP osteoporosis in rats,levels of Ca2+,significantly increased serum levels of BGP and P.May be initially identified Achyranthes steroidal component is the main active ingredient to treat osteoporosis in rats.Using the rat model of complete fracture,though X-ray observations,determination of biochemical indicators,rheology testing,the effects of Achyranthes split components on fracture healing in different periods of treatment.X-ray observations show that the two components can contribute to the healing of the fracture zone of fractures in rats,saponin component effect is more significant Biochemical examinations showed steroidal saponin constituent components and can be varying cldegrees of elevated serum ALP.BGP and Ca2+ levels,decreased serum P levels,including the role of Achyranthes steroidal components quick and lasting.Rheology test results found that plasma viscosity of saponin components significantly higher compared with the model group,overall changes in parameter values within the normal range.Show saponinc component is to play a major role in blood circulation effective component.Steroidal components and saponin components could significantly inhibit acetic acid-inducced rat paw edema in acute inflammatory exudate and egg white mice caused,Bidentata saponins rapid onset and longer duration of action,a significant anti-inflammatory effects in the swelling model.Dscription steroidal components and saponin components are Achyranthes promote effective component fracture healing,and steroidal saponin constituent components have a synergistic effect in the treatment of open fractures process.This study completed the process of investigation and study of Achyranthes split Bushenzhuanggu extracting effective parts,and validated steroidal saponin constituent components and do not cross the split was good.By in vivo pharmacological experiments identified Achyranthes steroid group was divided into the treatment of osteoporosis primary efficacy component.Steroidal components and saponin components all have a role in the treatment of fractures,showing a synergistic effect in the treatment of the fracture process.This study illustrates the pharmacological effects of Achyranthes effective part of the effective components,reflecting the traditional Chinese medicine,"a multi-component,multi-channel,multi-level,multi-target" effect characteristics.