| BackgroundPsoriasis is a chronic relapsing erythema scaly skin disease.Its clinical lesions is characteristic of erythema type scales.The main pathological features of psoriasis are epidermal hyperkeratosis with hyperkeratosis,acanthosis layer thickening,and the superficial layer of the capillaries of the dermis which is surrounded by inflammatory cells such as lymphocytes and neutrophils.The exact cause of psoriasis is not clear,more and more evidence showed that it had relation with genetic,environmental,immune and other factors.At present,most of the literatures suggest that psoriasis is a disease of Th17 cells and Thl cells,which is mainly regulated by Th17 cells.Th17 cell producing IL-17 is a kind of CD+4 effect T cell,which is different from Thl and Th2 in recent years.The JAK-STAT is the newly discovered pathway,which can transduce various cytokines.JAK,a non receptor protein tyrosine kinase(PTK),is also known as Janus kinase,which can is involved in regulation of cell such as proliferation,differentiation,apoptosis and other important biological activity.JAK family has four members:JAK1,JAK2,JAK3,TYK2,and JAK3 is now widely distributed in activated T cells,B cells and monocytes.What the the difference of peripheral blood Th17 cell factor IL-17 expression and Th17 expression of JAK3mRNA cells between psoriasis and healthy people?Whether IL-17 expression can be regarded as a sign of the active stage of psoriasis?Acitretin is the first-line drug for the treatment of psoriasis,and what kind impact of the Th17 cells in peripheral blood of patients with vulgaris psoriasis and IL-17 JAK3mRNA expression has after acitretin treatment?ObjectiveStudy the differences expression of IL-17 secreted by Th17 cells and JAK3mRNA in Th17 cells between psoriasis and healthy people.And then study the influence of IL-17 and JAK3mRNA in psoriasis after acitretin treatment,and provide a theoretical basis for clinical treatment of acitretin to psoriasi.MethodThere were 30 cases of patients with psoriasis vulgaris diagnosed in our hospital or Nanfang hospital,and control group has 30 cases from healthy people.and there were no significant differences in age and sex between the two groups(p<0.05).We were treated peripheral venous blood 2ml from Patients with psoriasis before and after treatment and control group and detected the expression of IL-17 by ELISA and detected 12 patients with psoriasis before and after treatment and control group by Rt-PCR method.We analysed data by SPSS20.0 statistical software.We could use two t test if the datas were measurement data and in accordance with the normal distribution;if not,use the Wilcoxon rank sum test;two-sided test,p<0.05 was statistically significant.ResultThe amount of blood IL-17 expression in psoriasis before treatment was higher than the healthy group,with statistical significance(t=-18.766,p<0.05).The expression of peripheral blood IL-17 in the experimental group after treatment was decreased than before treatment with statistical significance(Z=-4.762,p0.05),but IL-17 in the experimental group after treatment was also higher than the healthy group(Z=-3.408,p<0.05);The expression level of JAK3mRNA in psoriasis before treatment was higher than the healthy group with statistically significant(t=18.061,p<0.05).The expression level of JAK3mRNA in psoriasis before treatment was higher than the psoriasis after treatment with statistically significant(t=7.976,p<0.05),but JAK3mRNA in the experimental group after treatment was also higher than the healthy group(t=4.860,p<0.05).ConclusionIt is suggested that the pathogenesis of psoriasis was related to Th17 cells and its closely related cytokines especially IL-17.The mechanism of acitren in the treatment of psoriasis may be inhibit the important kinase JAK3 of JAK-STAT pathway,thereby inhibiting Th17 cell related cytokines secretion to reach certain therapeutic effect. |
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