| [Objective]This study aims to observe effects of the low frequency electromagnetic field(LFEMF)on the hair growth and the expression of KGF,TGF-?1,PDGF-BB and FGFR2 in hair growth cycle of mice.[Methods]120 hair natural growth model of C57BL/6 mice were randomly divided into magnetic field group and control group,60 in each group.Among them,the magnetic field group mice selected low-frequency electromagnetic field(50Hz,5 mT)exposure,30min/d,continuous intervention for 14 days.The control group did not do magnetic field treatment,but the feeding condition was consistent with the LFEMF group.After exposing the low frequency electromagnetic field for 3 days,7 days and 14 days,we harvested the skin specimens,and used the HE staining to assess for hair follicle histological morphology;RT-PCR to test the mRNA expression of KGF,TGF-?1,PDGF-BB and FGFR2;and test the protein expression of KGF,TGF-beta 1,PDGF-BB and receptor FGFR2 through Western Blot.[Results]1.We can see the hair in the 3d group was black,bright and thick;the hair in 7d group was falling off gradually and became thin;the hair in 14d group was almost fell off,and the skin turned pink.There was no significant difference in both LFEMF group and control group at 3d?7d.2.The results of HE staining:Compared with the control group,the length of hair was increased(P<0.05)in 3d and 7d after magnetic field intervention,and the thickness of mouse epidermis was thickened(P<0.05).When the magnetic field intervened on the 14th day,hair growth entered the departure,compared with the control group,its epidermis thickness was thicker.There was no significant difference in the growth cycle between the control group and the LFEMF group during the intervention.3.The results of Real-time PCR:In the LFEMF group and the control group,the expression of KGF at 3d,7d,14d was gradually decreased,but there was no significant difference((P>0.05);At the same time point compared with the control group,the expression of KGF mRNA group was significantly higher than that of the control group(P<0.05).In the control group,the pression of TGF-?1 at 3d,7d,14d increased,but there was no significant difference(P>0.05).The pression of TGF-?1 mRNA in the LFEMF group first increased,then decreased.The difference between the two groups with time points was not significant(P>0.05).The expression of PDGF-BB in the LFEMF group and control group were first increased and then decreased,but had no significant difference(P>0.05),The expression of PDGF-BB mRNA at different time points in the control decreased,but the difference was not significant(P>0.05);The expression of PDGF-BB at 3d and 7d were all higher than that of 14d(P<0.05).In the LFEMF group and the control group,the expression of FGFR2 at 3d,7d,14d was gradually decreased,but there was no significant difference((P>0.05);At 3d,7d,14d time point the expression of FGFR2 in the LFEMF field group was higher than that in control group,but the difference had no statistical significance(P>0.05);4.Western-blot test results:The expression of KGF protein in the LFEMF group increased slightly at each time point compared with the control group.The expression of KGF protein in the control group changed little with time,and the expressionroup of KGF protein at 7d in the magnetic field group was higher than that of 3d and 14d;the TGF-?1 protein expression changes little with time,but each time point LFEMF group was higher than the control group.At each time point,the expression of PDGF-BB is the LFEMF group was higher than control group;The expression in control group changed little at 3d and 7d,but the expression decreased at 14d,while the magnetic field group at 7 d expressd the most.The expression of FGFR2 in the LFEMF group was higher than control group;But there was no obvious change in the two group at 14d.[Conclusion]LFEMF can promote the growth of mouse hair in the growth cycle,In this process,LFEMF promotes the expression of growth factors KGF,TGF-?1,PDGF-BB and FGFR2. |
PDF Full Text Request