| BackgroundThe results of this study indicate that,Intrauterine growth restriction(Intrauterine growth restriction,IUGR)can cause serious adverse effects on brain function development in full-term infants.However,it is not clear whether the development of brain function is affected.Therefore,the research on the National Natural Science Foundation(No.81471087)supported by amplitude integrated electroencephalography(Amplitude integrated electroencephalogram,aEEG)on intrauterine growth restriction function were observed in preterm infants.On the basis of this study,the effects of taurine supplementation on the expression of miRNA in brain tissue of rats with fetal growth restriction were studied.The aim of this study was to provide some theoretical basis for prenatal supplementation of taurine to promote the development of fetal brain function.Part 1:Adverse effects of intrauterine growth restriction on the development of preterm fetal cerebral functionObjective To determine the adverse effects of intrauterine growth restriction(IUGR)on the development of preterm fetal cerebral function.Methods A total of 110 preterm infants with gestational ages from 32 to 36+6 weeks were collected in the Ba Yi Children’s Hospital Affiliated to Beijing Army General Hospital during the period of Jan.2015 to Feb.2016,including 50 SGA infants and 60 AGA inrants The amplitude-integrated electroencephalography(aEEG)recordings were obtained within 72 h after birth by using the NicoletOne monitor.Duration of each recording was 4 to 6 hours in each premature infant.The observational indices included continuity,sleep wake cycling(SWC),interburstinterval(IBI),minimum voltage and maximum voltage.The Student’s t test,Chi-squared test and analyses of variance were used to compare differences between the two groups.Results The frequency of aEEG continuity:SGAgroups<AGAgroups(p=0.002),SWC:SGAgroups<AGAgroups(p=0.002).IBI:SGAgroups>AGAgroups(p=0.000).Maximum voltage:SGAgroups<AGAgroups(p=0.002).Minimum voltage:SGAgroups<AGAgroups(p=0.002).The difference was statistically significan(p<0.05)t.The birth weight of SGA group was less than 3%,3%～5%,and 5%～10%there was no significant difference(p>0.05).Hypoglycemia(without hypoglycemic encephalopathy)or hyperbilirubinemia(without bilirubin encephalopathy)had no significant influence on fetal cerebral function(p>0.05).Conclusion SGA infants have a poor occurrence rate of aEEG continuity and SWC,longer IBI,and lower maximum and minimum voltage,which suggests that SGA,to an extent,has a delayed or adverse influence on the development of preterm fetal cerebral function.Part 2:A pilot study on the Effects of antenatal taurine on the expression of miRNA in fetal rat brain tissue with growth restrictionObjective:To observe the differential expression of small RNA(miRNA)in brain tissue of fetal rats with intrauterine growth restriction and prenatal taurine supplementation.To explore the effect of miRNA on fetal brain injury associated with intrauterine growth restriction,and the effect of prenatal taurine supplementation on the expression of miRNA in brain tissue of rats with fetal growth restriction.Methods:24 healthy Sprague-Dawley pregnant rats were randomly divided into 3 groups:control group,FGR group,taurine group,each group of 8 pregnant rats,according to the whole process of FGR.The expression of miRNA in brain tissue ofFGR group and taurine group was screened by microRNA PCR chip detection technology(including real-time fluorescent quantitative PCR(RT-PCR)).Results:In the FGR group and taurine group,we screened a number of differentially expressed miRNA.By comparing taurine group with control group,there were 18 differentially expressed miRNAs,5 of which had significant difference(P<0.05):mmu-mir-714,miR-135b,let-7b,miR-325,miR-148a*.By comparingFGR group with control group,there were 35 differentially expressed miRNAs,12 of which had significant difference(P<0.05):miR-223,mir-875-3p,mir-299*,miR-450a-5p,mmu-mir-714,miR-135b,mir-491,miR-302b,miR-325,miR-26b,miR-340-3p,miR-208b.By comparing taurine group with FGRgroup,there were 16 differentially expressed miRNAs,3 of which had significant difference(P<0.05):miR-223,miR-30c,mir-211。According to the difference of the expression level of miRNA gene in taurine group,control group and FGRgroup,it was found that the expression level of miR-223 in the 3 groups was statistically significant(P<0.05):taurine group>control group>FGRgroup.Conclusion:According to the above results suggest that mir-875-3p,mir-299*,miR-450a-5p,mmu-mir-714,miR-135b,mir-491,miR-302b,miR-325,miR-26b,miR-340-3p,miR-208b,miR-223 may be involved in the process of intrauterine growth restriction of brain damage.Mmu-mir-714,miR-135b,let-7b,miR-325,and miR-148a*may be involved in the process of improving fetal brain damage induced by taurine.Prenatal supplementation of taurine may promote the expression of miR-223 in fetal brain tissue of fetal rats with intrauterine growth restriction,and intrauterine growth restriction may inhibit the expression of miR-223. |
