Effect Of Burdock Root Aqueous Extract On TLR4/NF-?B Expression In Vascular Endothelial Cells Protection In Patients With Hypertension

Objective:To explore the effect of burdock root aqueous extract on toll-like receptor 4-nuclear factor ?B(TLR-NF-?B)signal pathway in vascular endothelial cells in patients with hypertension.Methods:From December 2014 to January 2016,we enrolled 40 patients of essential hypertension in the Hospital(the hypertension group)and 40 cases healthy individuals(the control group).Human umbilical vein endothelial cells(HUVEC-C)were induced with serum collected from the two groups of subjects for 24 h.Different doses of burdock root aqueous extract were used to intervene TLR4 expression(24h)and activation(2h)in HUVEC-C induced by serum from patients with hypertension.The cells were continuously cultured for 24 h,and then the protein was extracted.The expression of TLR4 protein and NF-?B inhibitor I-?B? was detected by Western blot.Results:(1)The TLR-4 level in the hypertension group was significantly higher than that in the control group,(0.6683±0.07468)vs(0.2220±0.02749),while I-?B? level in the hypertension group was significantly lower,(0.2687±0.02519)vs(0.5616±0.07762),with statistically significant differences(P<0.05).(2)The TLR-4 levels in the intervention groups with low-dose,middle-dose and high-dose burdock root aqueous extract were(0.2720±0.05178),(0.4509±0.03399),(0.3810±0.07796),which were significantly lower than(0.6683±0.07468)in hypertension group and significantly higher than(0.2220±0.02749)in the control group.The I-?B? levels in the intervention groups with low-dose,middle-dose,and high-dose burdock root aqueous extract were(0.5059±0.03042),(0.3433±0.02289),(0.3653±0.00710),respectively,which were significantly higher than(0.2687±0.02519)in the hypertension group and significantly lower than(0.5616±0.07762)in the control group.The above differences were all statistically significant(P<0.05).Conclusions : Immune disorders and inflammatory response mediated by TLR-NF-?B signal pathways may be one of the mechanisms of vascular endothelial injury in hypertensive disease.Burdock root aqueous extract can inhibit the expression of TLR-4,NF-?B,thereby inhibiting the expression of inflammatory cytokines to protect vascular endothelial cells.