| Objectives:1.To observe the effct of rats lack of Vitmin D during pregnncy and lactation on the weight?lung weight and lung developmentof offspring rats.2.To detect the expression of CyclinD1 in rats lung tissue in different groups.3.Study the effects of vitamin D deficiency on rat lung development on offspring of the possible mechanism.Methods:The adult female Sprague-Dawley rats were randomly assigned to vitamin D deficiency group(VDD group)and normal control group(control group),a total of 6rats in each group,VDD group avoided light completely and fed by fodder which do not contain vitamin D,control group had the normal illumination and general feed,2weeks later,the female rats copulated with the adult male SD rats in the same cage with 2-1 ratio,and then we established a model of VitD deficiency of pregnant rats.By using competitive enzyme-linked immunosorbent assay(ELISA)technology to detect the levels of serum 25-(OH)-D3 of the female rats after postpartum 21 days.Respectively took lung tissue of the 1,7,14,21 days after offspring rat borned,then observed the lung tissue morphological structure by microscope respectively,at the same time,detected the expression of CyclinD1 in each lung tissue by immunohistochemical method.Results:1.The level of serum 25(OH)D3:Two groups try feeding after 2 weeks,the difference of VDD group and the control group of serum 25(OH)D3 level had no statistical significance(P > 0.05);In female postpartum 21 days,VDD groups of serum 25(OH)2 D3 levels lower than the control group,the difference is statistically significant(P < 0.05);VDD group on 21 postpartum days significantly lower than try feeding after the two weeks,the difference was statistically significant(P < 0.05);Feed 2 weeks and postpartum 21 days of the control group,the levels of serum 25(OH)-D3 had no statistically significant difference(P > 0.05).2.The level of weight: the body weight of offspring rats in VDD group and the control group increase gradually,but difference is no statistical significance(P >0.05).3.The level of lung weight: offspring rat lung wet weight in VDD group and control group increase gradually,postpartum 7,14,21 days lung weight VDD group is lower than the control group,the difference is statistically significant(P < 0.05),postpartum 1 day lung weight similar between the two groups has no statistical significance(P > 0.05).4.The difference of the offspring rats lung tissue in microscope: the lung tissue structure observed on the same day of VDD group was wider alveolar septa,less alveolar tissue,smaller and irregular alveoli,irregular terminal bronchioles and small bronchi than control group.3.The expression of the CyclinD1: VDD group and control group each in the bronchial mucosa epithelial cells express CyclinD1 clearly,the control group expressed CyclinD1 strongest in 1st day after the birth of the newborn rats,and waned in the 7th,14 th,21st days.But,the VDD group expressed CyclinD1 strongest in 7th day after birth,higher than the control group(P < 0.05),the 1st day expression was lower than the control group(P < 0.05),the 14 th,21st days expression were higher than the control group(P < 0.05).Conclusion:1.Lack of vitamin D during pregnncy and lactation of mother rats have little little influence on the lung wet weight and postpartum 1 daylung weight,but reduce the postpartum 7,14,21 days lung wet weight of offspring rats.2.Lack of Vitmin D during pregnncy and lactation of mother rats delayed the lung developmentof offspring rats.3.Mother rats during pregnancy and lactation lack of VitD delayed expression of CyclinD1 peak in offspring rat lung tissue and delayed the attenuation process of CyclinD1 expression.4.CyclinD1 participate in lung tissue cell proliferation,one of the possible mechanisms of the female rats inhibite offspring rats lung development is likely to bevitamin D deficiency delay expression of CyclinD1. |
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