Study On Oxidative Stress And JWA MRNA Expression In Myocardial Injury Of Iron Overloaded Male Mice

Objective: Iron is one of the most important trace elements in the human body and has many important biological functions.Iron is involved in the process of protein synthesis,cell respiration and basic expression regulation.It is easy to cause multiple organ damage when the body iron overload,and the heart is damaged one of the main target organs.JWA acts as a new active environmental response gene that protects cells from reactive DNA-related DNA damage.This study was to investigate the expression of JWA gene mRNA in male mice under iron overload,and to explore the effect of iron overload on the expression of JWA gene mRNA in myocardial tissue of male mice and its correlation with oxidative stress injury.Methods: Forty male rats were randomly divided into 4 groups(control group and 3 different dose of iron overload group)according to body weight.Each group was injected with different doses(0mg / kg · bw,50 mg / kg · bw,100 mg / kg · bw,150 mg / kg · bw),and the rats were sacrificed for 4 weeks.The rats were sacrificed and the liver tissues were sacrificed.The expression of JWA gene mRNA in myocardium was measured by polymerase chain reaction(RT-PCR).The levels of malondialdehyde(MDA),catalase(CAT),hydrogen peroxide(H2O2)and glutathione peroxidase(GSH-PX).Results: Compared with the control group,the iron content of serum iron and liver tissue of three groups of different doses of iron overload mice were significantly increased(P<0.05);The expression of JWA gene mRNA in the myocardium of 100 mg /kg · bw dose group and 150mg/kg · bw dose group was significantly higher than that in the control group,and the difference was statistically significant(P<0.05).The expression of JWA gene mRNA in myocardial tissue was negatively correlated with CAT activity,H2O2 content and GSH-PX level,but positively correlated with MDA content and hydroxyl free radical activity(P<0.05).Compared with the control group,the content of MDA and the activity of hydroxyl radicals in the three groups were significantly higher than those in the control group(P <0.05),while the activities of CAT,H2O2,and GSH-PX were decreased(P <0.05).Conclusion: Iron overload leads to high expression of JWA gene mRNA in mouse myocardium and is closely related to oxidative stress injury in myocardial tissue.