Establishment And Application Of Volatile Organic Compounds In Human Hematuria

In order to master the basic situation of the main environmental pollution impact on the health of the population in key areas of China,to assess the health risks caused by environmental pollution,the Ministry of Environmental Protection will jointly organize the "National Key Laboratory of Environmental and Health" in conjunction with the State Health Commission in 2015-2017 "(Hereinafter referred to as" special survey "),This study is part of a special survey,which is responsible for the establishment of methods for the detection of volatile organic compounds in blood and laboratory quality control.Objective To establish a method for the detection of 13 volatile organic compounds(VOCs)in blood and urine.Methods Purification was carried out by purging and trapping-GC-MS and solid-phase microextraction-GC-MS.The samples were treated with defoamer and isotope internal standard.The samples were prepared under high purity nitrogen atmosphere.The results showed that the volatile organic compounds were detected by gas chromatography/mass spectrometry and gas chromatography/mass spectrometry and isotope internal standard method.The volatile organic compounds were detected by solid phase microextraction-gas chromatography/mass spectrometry-isotope internal standard method.Detection method.Results In the range of 0.045 ng/mL-5.76 ng/mL concentration,the linear coefficients were more than 0.995 and the blood sample volume was 5.0 mL when the 13 target compounds were detected by purging and trapping-The detection limit was 0.03 ng/mL-0.58 ng/mL,the recoveries were 81.4%-117.8%and the RSD was 2.5%-12.3%.When the urine sample volume was 30.0 mL,the detection limit was 0.006 ng/mL-0.15 ng/mL,the recoveries were 76.3%-105.1%and the RSD was 1.7%-11.2%.The linear coefficient was more than 0.995 in the concentration range of 0.045 ng/mL-5.76 ng/mL when the 13 kinds of target compounds in blood samples were detected by solid phase microextraction and GC/MS.When the blood sample volume was 5.0 mL The detection limit was 0.03 ng/mL-0.58 ng/mL,the recoveries were 84.4%-121.9%and the RSD was 1.7%-12.5%.Using purge and trap gas chromatography-mass spectrometry detection of volatile organic compounds in 31 blood samples,the xylene concentration of 0.091 ng/mL-0.194 ng/mL ng/mL-0.189 ng/mL,ethylbenzene concentration was 0.099,the concentration of toluene was 0.492 ng/mL-1.512 ng/mL 0.072 ng/mL-1.355 ng/mL,the concentration of benzene,p-xylene concentration of 0.172 ng/mL-4.152 between ng/mL.Using purge and trap gas chromatography-mass spectrometry detection of volatile organic compounds in 38 urine samples,the xylene concentration in the range of 0.023 ng/mL-0.214 ng/mL,ethylbenzene concentration in the range of 0.101 ng/mL-0.218 ng/mL,the concentration of toluene in the range of 0.034 ng/mL-0.124 ng/mL,between the p-xylene concentration in the range of 0.089 ng/mL-0.215 ng/mL.Determination of 9 volatile organic compounds in blood using solid-phase microextraction,o-xylene concentration in the range of 0.147 ng/mL-0.241 ng/mL,the concentration of toluene in the range of 0.114 ng/mL-0.257 ng/mL,the concentration of benzene was 0.306 ng/mL-0.324ng/mL,between the p-xylene concentration in the range of 0.145 ng/mL-0.147 ng/mL.Conclusion This study solves the problem that the blood and urine are clogged during the purging process,reduces the detection blank and reduces the matrix effect.It is suitable for the routine detection of volatile organic compounds in large amount of blood and urine samples.The method is simple and efficient.The detection method established by solid phase microextraction-gas chromatography combined with high efficiency is simple and easy to operate.