The Research Of SRPK2 On The Invasion, Migration And Angiogenesis Of Glioma Cells

Objective: To study whether SRPK2 can have high expression in gliomas and promote the invasion, migration and angiogenesis of gliomas cells.Method: First, cultivate HA1800, U251 and U87MG cell line, and the expressio n of SRPK2 in normal glial cells and glioma cells were detected by immunofluoresce nee and Western Blotrespectively. For the further study of the effect of SRPK2 on the glioma cells, we use flow cytometry to detect infection efficiency after glioma cells w ere infected by virus.And infection effect was detected by RT-PCR and Western Blot.The growth and apoptosis effect of SRPK2 on glioma under the in vitro and in vivo c ondition were respectively detected by flow cytometry and nude mouse tumorigenicit y assay. The invasion and migration effect of SRPK2 on glioma were detected by Tra nswell Chamber and cytoskeleton staining.The effect of SRPK2 on angiogenesis were measured by small tube forming experiment. The effect of SRPK2 on ?-catenin were measured by by Western Blot in vitro and in vivo condition.Results: Immunofluorescence and Western Blot results showed that SRPK2 has high expression in glioma cells and low expression in normal glial cells.Glioma cells were infected with appropriate MOI under the detection of flow cytometry in order to reach the highest infection efficiency, and the best virus-infection glioma cells were selected by RT-PCR and Western Blot. And flow cytometry and nude mouse tumorigenicity assay suggested that SRPK2 can promote the growth of glioma cells and inhibit its apoptosis. Transwell Chambers and cytoskeleton stainingresults demonstrated that SRPK2 can enhance the invasion and migration ability of glioma cells by adjusting the number and arrangement of actin. Small tube forming experiments showed that SRPK2 can promote tumor angiogenesis.The Western Blot results in vitro and in vivo condition showed that SRPK2 can promote the expression of p-catenin.Conclusion: SRPK2 can promote the growth of glioma cell, inhibit its apoptos is, and enhance its invasion and migration ability. SRPK2 can also promote angiogene sis of the glioma. SRPK2 can play a role in glioma because of the high expression of?-catenin in the pathway of Wnt/?-catenin-catenin by regulating the SR protein.