Effect Of Modified Zhishixiebaiguizhi Decoction On Over Oxidize Injury In Myocardial Ischemia Reperfusion Rats

ObjectiveThe aim of this study is to investigate the suppress effect of Modified Zhishixiebaiguizhi Docoction on over oxidize injury for myocardial ischemia/reperfusion rats and its possible mechanisms.Methods60 healthy male SD rats were randomly divided into 4 groups,control group,model group,Zhishixiebaiguizhi Docoction group and Modified Zhishixiebaiguizhi Docoction group,each group of 15 rats.Adaptability to feed for a week,the drug groups respectively according to 4.59 g/kg and 6.48 g/kg dose lavage Zhishixiebaiguizhi Docoction and Modified Zhishixiebaiguizhi Docoction,the rest of the groups lavage isopyknic distilled water,1 times a day for 14 days.Then use the LAD ligation ischemia for 30 min,and reperfusion for120 min,to set up the myocardial ischemia reperfusion injury,while The sham group thoracotomy without ligation.Evan's blue-TTC double staining method to detect myocardial infarction areas,HE staining to watch morphological changes,Detection of NO,MDA,SOD contents in serum and MDA,SOD contents in myocardial tissue by Elisa,p-e NOS protein expression changes by immunohistochemical,and detect Akt,p-Akt,e NOS,p-e NOS,c GK1 protein contents in myocardial tissue by western blot method.ResultsCompared with sham operation group,the model group's cardiac infarct size was increased(P<0.01),Myocardial morphology disordered,the MDA of serum and tissue were increased,the NO,SOD of serum and tissue were decreased,the p-Akt,p-e NOS and c GK1 of myocardium tissue weredecreased(P<0.01),Compared with model group,the two drug groups cardiac infarct sizes were decreased(P<0.01),Myocardial tissue morphology change smaller,the MDA of serum and tissue were decreased,NO,SOD of serum and tissue were increased,the p-Akt,p-e NOS and c GK1 of myocardium tissue were increased(P<0.01),Compared with Zhishixiebaiguizhi Docoction group,Modified Zhishixiebaiguizhi Docoction group infarct sizes were decreased(P<0.05),the NO of serum and the p-Akt,p-e NOS of myocardium tissue were increased(P<0.05),the others were no significant difference(P>0.05).ConclusionModified Zhishi Xiebai Guizhi Docoction has inhibitory effect of myocardial infarction,increseased the production of blood vessels NO,decreased the myocardium over oxide injury.The mechanism may related to PI3K-Akt-e NOS up-regulated NO-c GMP-PKG signal pathway.