Effects Of JAK/STAT Signaling Pathway On Migration And Proliferation Of Human Hepatocellular Carcinoma HepG2 Cells Induced By Morphine

Objectives : To survey the influence of morphine on migration,proliferation and expression of STAT3 and STAT5 mRNA in human hepatocellular carcinoma HepG2 cells.Methods:Containing 10umol/L of morphine(group B),50 umol/LAG490(group C)and 10 umol/L morphine combined with 50 mol/LAG490(group D)cell culture medium,were incubated with human hepatocellular carcinoma Hep G2 cell 48 h,and setting a blank control group.The wound healing assay was put used to observe the migration activity of HepG2 cells.We used the MTT method to detect HepG2 cells proliferation when HepG2 cells was incubated for 48 h.The expression of STAT3 and STAT5 mRNA was tested by using semi—quantitative reverse transcription polymerase chain reaction(RT—PCR).Results:1?Scratch injury 48 hours later,the drug treatment group(include group B,group C and group D)of the healing rate was lower than the control group,the ability of cell migration was clear inhibited,the diversity was statistically significant(P<0.05);Group D compared with group B and group C,the migration ability decreased more(P<0.05).2?During the MTT assay of human hepatocellular carcinoma HepG2 cells proliferation ability.The results show that compared with the control group was measured,the measured absorbance of group B,group C and group D reduced significantly,and the disparity was statistically significant(P<0.05);Group D compared with the group B and group C,the absorbance reduced(P<0.05).3?The results of RT-qPCR showed that group B,group C and group D of STAT3 mRNA in human hepatocellular carcinoma HepG2 cells expression was measured,it was clear reductive compared with the control group,and the diversity was statistically significant(P<0.05).Besides compared with group B and group C,the expression of group D was reduced(P<0.05);STAT5 mRNA in group B and group D was significantly decreased compared with the control group(P<0.05),group C STAT5 mRNA expression compared with the control group reductive,but the diversity was not statistically significant(P>0.05),The expression of mRNA STAT5 in group D compared with group C,it was significantly decreased(P<0.05).Conclusion:1?Morphine can inhibit human hepatocellular carcinoma Hep G2 cells migration and proliferation ability,and its mechanism may be related to the inhibition of JAK/STAT signaling pathway and down-regulation of expression of STAT3 and STAT5 gene,2?AG490 can enhance the inhibitory action of morphine on migration of human hepatocellular carcinoma HepG2 cells and value-added capabilities.