| Gastrointestinal cancer is one of the most prevalent diseases in the world,leading to more than 40 percent morbidity and mortality in all cancers.In today's medical means for the diagnosis of gastric cancer and colorectal cancer is still mostly stay in the physical means of the level,and in the early gastrointestinal diseases in cancer without special physiological symptoms to be extremely difficult to find in the early.In this study,blood samples from 100 healthy volunteers and blood samples from 14 gastric cancer patients and 14 colorectal cancer patients were collected.Three blood cells were extracted from the blood,and the phospholipids on the cell membrane were extracted and the fatty acids were derived To obtain a good volatile fatty acid methyl ester,you can use the temperament to detect the content of fatty acids.In the study of lipid group,based on the three blood cells extracted from the blood,this experimental method rarely reported in metabolomics.Because the three metabolic patterns of blood cells are different,the shortest life of white blood cells is about 10-12 hours,the longest life of red blood cells,but mature red blood cells can not own synthetic fatty acids can only rely on external absorption,platelet life is about 8-11,So the three kinds of cell membrane can be obtained on the fatty acid metabolism of information is of great significance.For the healthy control group,100 samples were tested in the experiment,and the accuracy and stability of the test method were tested by mean value,variance,skewness and kurtosis.Then the samples of gastric cancer patients and colon cancer patients were tested.The data of fatty acid were analyzed and analyzed by SIMCA-P and SPSS.Different types of fatty acid differential metabolites are found in each cell membrane of the two diseases,so it is very valuable to separate and extract blood cells.In the analysis of gastric cancer,the significant differences in metabolites were screened out through the analysis of the box diagram to detect the normal data range of healthy people and gastric cancer patients with the intersection of fatty acids,respectively,about gastric cancer,C18-1,C20-5 in the white blood cells,C20-5,C20-3n3 in erythrocytes,C20-5 on platelet membrane.The two fatty acids on the blood cell membrane of the gastric cancer also appear in the differential metabolites of the three blood cells,which are C20-5 and C20-2,respectively.As the C20-5 data range distribution is not compact,very large variance.Compared with the C20-2 data distribution not only and healthy people did not intersect,and the distribution is very uniform,within a fixed range of small variance,and the healthy control group mean difference is very large,it is a very good screening of the difference and could be used in the diagnosis and treatment of gastric cancer as an important target or reference object on three cell membranes.In the colorectal cancer cell membrane,white blood cells and platelets the test results contained C22-ln9 at the same time,but the white blood cell data range distribution is not compact,and with great variance;while the data in the platelet distribution is not compact but healthy people There is no intersection,and the difference is very obvious,there is no interference in the intersection.Simultaneous analysis of red blood cells in the four fatty acids,there are significant differences,although the value of close but less interference.So C22-ln9 in platelets and C12-0,C14-0,C20-3n3 and C22-6 on erythrocyte membrane can be an important target in the diagnosis and treatment of colorectal cancer as an important target on colorectal cancer cell membrane. |
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