Effects Of Graphene Coating On Sructure And Function Of Red Blood Cell

In order to obtain Graphene oxide (GO) with good biocompatibility and expand its application in biomedicine, in this experiment oxidized graphene was synthesized from oxidized graphite by using oxidation-reduction method(Hummers method). X-ray photoelectron spectroscopy (XPS), Transmission ELectron Microscopy (TEM), Atomic Force Microscope (AFM), and Infrared Radiation (IR) were used to characterize the structure of graphene oxide synthesized. The results showed that a single layer of graphene oxide with thickness of about 1 nm was successfully synthesized by oxidation-reduction method.The RGO, BSA dispersed GO and Tween dispersed GO were obtained by reducing and dispersing the oxidized graphene in the experiment, respectively.The GO, RGO, BSA dispersed GO and Tween dispersed GO were studied for cytotoxicity. The results show that the oxidized graphene has better hemocompatibility than that of the reduced and dispersed graphene oxide.Compared with the oxidized graphene, Tween-dispersed oxidized graphene had better hemocompatibility and less cytotoxicity. The fragility of erythrocytes is mainly affected by the dispersant, pfl27 dispersed graphene showed a certain reduction of red blood cell brittleness protection, BSA dispersed graphene on the red blood cell fragility almost no effect. Graphene encapsulation, reduce the red blood cell deformation rate, showing a certain role in the protection of red blood cells.CCK8 experiments were carried out on the toxicity of graphene to different spleen cells of different sizes at different concentrations. The results showed that the toxicity of graphene oxide to cells was greater than that of 200 mesh and 1000 mesh graphene. The toxicity and size of graphene on lymphocytes directly affect their cytotoxicity, and there is a significant concentration-dependent. Different sizes of graphene oxide, the cytotoxicity decreased significantly with size; GO size of the larger, only below a certain concentration to show good biocompatibility. In the gravimetric oxide in the size of 200 mesh, graphene on the cells have a protective effect, cell activity reached 125%. By performing a flow cytometry, it is possible to obtain a simple cell sorting of enriched graphene at a concentration of 5 ?g/mL graphene size at 1000 mesh. 200-purpose graphene oxide has little effect on cell screening. The fluorescence intensity of CD 19 PE + cells increased with the increase of the concentration of graphene, and the fluorescence intensity of CD3 PE + cells increased gradually. The fluorescence intensity of CD3e FITC+ was decreased and the fluorescence intensity of CD3e + It was judged that the oxidized graphene enriched the fluorescence of FITC, thereby reducing the fluorescence intensity of FITC.