Standardization Research Of Buyanghuanwu Decoction

This subject comes from the State Administration of Traditional Chinese Medicine in the pharmaceutical industry research and special "Standardization technology research of Clinical Chinese medicine decoction".Buyanghuanwu Decoction(BYHWD)is from "Correction of Medicine",which was created by the Qing Dynasty famous doctor Qingren Wang.It is composed by astragalus(120g),angelica tail(6g),red peony root(5g),earthworm(3g),Chuanxiong(3g),peach kernel(3g)and safflower(3g).BYHWD can promote blood flow and circulate meridians,which is a famous prescription to cure hemiplegia and wilt.It has a good effect on cerebrovascular accident,polio sequelae,bust paralysis and atrophy of soft.Chinese medicine decoction specifications generally absent,decoction are both from the traditional boiling and the machine does not have a strict rules,machine decoction bags used for chemical material,boiling at high temperaturessecurity is urgently needed research.Therefore,this subject study for BYHWD,comparatively study of their the traditional technology and modern machinery technology,preferably the best boiling process to ensure its quality and clinical efficacy.It is reliable assurance and a strong basis for clinical Chinese medicine decoction technology standardized.This research mainly including the following several aspects:Literature Access to the ancient,modern and foreign literature,the writer explore the technical standardization of traditional decoction and machine decoction,study traditional decoction of the main factors,advantages and disadvantages of the machine decoction,discuss the problems that the traditional decoction and machine decoction faced.Summarized the recent progress of BYHWD,to understand the chemical composition of the herbs in the BYHWD and modern clinical application.BYHWD traditional boiling process research Single factor experiments were carried out on the factors of BYHWD boiling,and considered the combination with the clinical practice,establishing an Orthogonal Design of BYHWD to study traditional decoction process,the results of the experiments are analyzed by direct and variance analysis,to determine the best traditional boiling process of the BYHWD:plusing 10 times the total quantity of water,soaking 30 minutes,and boiling 2 times,each time was 30 minutes,and concentrated to 300 mL.Research boiling effect of BYHWD by using the different decoction appliances(casserole,stainless steel pot,and health pot),and determine the best appliance casserole.BYHWD modern boiling process research Firstly,single factor experiments were carried out on the factors of BYHWD machine boiling,and considered the combination with the clinical practice,establishing Orthogonal Designs of BYHWD to study atmospheric machine decoction process and the high-pressure,the results of the experiments are analyzed by direct and variance analysis,to determine the best machine boiling process of the BYHWD.Atmospheric:plusing 12 times the total quantity of water,soaking 30 minutes,and boiling 2 times,each time was 30 minutes;high-pressure:plusing 12 times the total quantity of water,soaking 30 minutes,and boiling 60 minutes,the temperature is 120℃.Secondly,research the impact about the BYHWD quality which had concentrated,it found that concentrated to 200mL-400mL compared with no concentrated group have no significant impact.Finally,research the machine boiling wrapping materials for BYHWD.Nowadays,the mechanical boiling is widely used in decoction of Chinese medicine.People usually put Chinese medicine into decocting bag in boiling to prevent machine clogging,so the bag use frequently.According to the survey,the decocting bag is varietious,the material is mostly made of non-woven fabrics and cotton.In this subject,experiments were carried out to research different wrapping materials.Finally,cotton was found to be the best wrapping materials.Simultaneous Determination of 6 compounds in BYHWD by HPLC To establish an HPLC method for simultaneous determination of amygdalin,paeoniflorin,ferulic acid,calycosin glucosidase,quercetin and formononetin in BYHWD.It developed a method for simultaneous determination of 6 ingredients in BYHWD.The method is simple,sensitive,accurate and reproductive,which provides a reliable detection methonds for quality control of BYHWD.Pharmacodynamic tesearch of BYHWD In this subject,to give hyperlipidemia rats Xuezhikang suspension and BYHWD liquid which were made by the traditional boiling and machine boiling.The results showed that blood viscosity and blood lipids in the model group significantly increased.After administration,the positive drug group,traditional groupand machine group have lower blood viscosity and lipids.The positive group effect is significant,traditional group and machine group is no significant difference.In summary,BYHWD is widely used as a clinical decoction.It is a very good representation to have a scientific research about traditional decoction and machine decoction.The subject,through optimum process preferred traditional decoction and machinery decoction of BYHWD.Study the wrapping material.So it will be more scientific,effective to control of quality about BYHWD.It provide a reliable basis and strong guarantee for the development of standards of clinical decoction standardized.