MicroRNA Expression Profiling Following Administration Of Dexmedetomidine In Rat's Heart

Background and objective:The use of dexmedetomidine may have benefits on the clinical outcomes of cardiac surgery.MicroRNAs are non-coding RNA sequences that act as regulators of gene expression.We conduct a study to determine the role of microRNAs in cardioprotection mediated by dexmedetomidine.Methods:In this study 6 rats were selected and they were divided into two groups.They are dexmedetomidine group and control group.Those rats were anesthetized.In dexmedetomidine group,those rats(n=3)were given intraperitoneally dexmedetomidine in a dose of 100?g/kg whereas in other group(n=3)normal saline of lml were administer intraperitoneally.Thirty minutes after administration,the hearts in both groups were excised and was stored in the liquid nitrogen and both the samples were marked.Those sample were analyzed for differentially expressed microRNA with Exiqon miRNA Array.Result:MicroRNAs were found to be differentially expressed and found that 5 microRNAs(rno-miRNA-X2,rno-miRNA-X3,rno-miRNA-X4,rno-miRNA-X5 and rno-miRNA-X6)were upregulated and one microRNA(mo-miRNA-X1)was found to be down regulated between two groups.MicroRNAs from control were compared with microRNAs of dexmedetomidine and were statistically evaluated using T-test in which all the P value was less than 0.05(P<0.05).This shows that all the above result was statistically significant.Conclusion:The use of dexmedetomidine is associated with differentially expressed microRNAs which may be involved in cardioprotection following dexmedetoidine administration.