| BackgroundAmplified in Breast cancer 1(AIB1)is a gene which is found by anzick SL in 1997 in searching for frequently amplified genes in breast cancer,which belongs to a member of the p160 the steroid receptor coactivator(SRC)family.Previous studies have shown that AIB1 constitute a complex signal transduction pathway with its transcription factors,and it plays an important role in the body’s growth and development,metabolism and endocrine regulation,and it also involves in the occurrence of many tumors,the development,invasion and transfer process in tumor.Epithelial Mesenchymal Transition(EMT),refers to the transformation of epithelial cells into stromal cells under physiological and pathological conditions.It has been shown that the EMT process of cells is involved in hepatocellular invasion and metastasis.It is key in the invasion and metastasis of tumor.The experiment was designed to detect the expression of AIBl and epithelial stromal transform(EMT)markers in human hepatocellular carcinoma tissuses and adjacent tissues,and explore the relationship between clinicopathological features of human hepatocellular carcinoma and the expression of AIBl and epithelial stromal transform markers.ObjectiveThis study was to detect the expression of AIBl and epithelial-mesenchymal transition markers in hepatocellular carcinoma tissuses and adjacent tissues.And the detection helped to further analyze the relationship between clinicopathological features of human hepatocellular carcinoma and the expression of AIBl and epithelial stromal transform markers.It aimed to detect the effect of AIB1 by RNA interference onexpression of EMT markers and invasiveness of HepG2.MethodsIn this study,the expression of AIBl,ZO-1,E-cadherin,Vimentin and N-cadherin protein in 85 hepatocellular carcinomas assessed through immunohistochemistry and clinicopathologocal significance was analyzed.After the lentiviral vector of AIB1 RNA interference was transfected in to HepG2 cells,the expression of AIB1 and EMT markers was detected by real-time PCR and Western blot.And the invasion of these cells was evaluated by Transwell analysis.ResultsThe expression of AIBl and Vimentin protein in hepatocellular carcinoma was significantly higher than that in normal adjacent tissues,and the expression of E-cadherin protein was lower than that of normal adjacent tissues.There was a significant difference in the frequency of AIB1 overexpression and the frequency of AIB1 overexpression(63% vs 43%,P <0.05)in hepatocellular carcinoma with lymph node metastasis.(66%vs 19%,P <0.05).The frequency of low expression of E-cadherin was significantly higher than that of non-lymph node metastasis(P<0.05),and the frequency of Vimentin protein expression was significantly higher than that of non-lymph node metastasis of E-cadherin(69% vs 43%,P <0.05).Correlation analysis demonstrated that the AIB1 protein expression was inversely correlated with E-cadherin(r =-0.435,P <0.05),and positively correlated with Vimentin in hepatocellular carcinomas(r =0.358,P<0.05).The expression of AIBl m RNA in HepG2 cells was significantly decreased after pLenti6-AIBl RNAi lentiviral infected HepG2 cells.The invasion of HepG2 cells was significantly descented.The expression of E-cadherin protein was significantly up-regulated and the expression of Vimentin protein was increasingly declined.ConclusionCompared with the normal adjacent tissue,the expression of AIB1 protein was significantly up-regulated in the hepatocellular carcinoma tissue.The expression of AIB1 gene in HepG2 cells could decrease the expression of Vimentin protein and increase theexpression of E-cadherin protein in hepatocellular carcinoma cell HepG2.The expression of AIB1 might promote invasiveness and metastasis of cancer cells in hepatocellular carcinomas. |
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