Research Of The Chemical Separation And Pharmacological Effects For Promoting Fracture Healing Effective Parts Of Sambucus Williamsii Hance

In the identified Sambucus Williamsii H.promoting fracture healing,on the basis of effective parts and its main bioactive this experiment elder effective docking site for chemical resolution,at the same time from the effective part and effective components of the elder was two chemical levels a systematic research on the pharmacology.Clarified the Sambucus williamsii H.efficacy material base and pharmacological effects at all levels.To make full use of Sambucus williamsii H.abundant natural resources provide an important basis.1.Research of the chemical separation and do not cross resistance for promoting fracture healing effective parts of Sambucus williamsii H.Using Sephadex LH-20 gel column chromatography and docking elder components in promoting fracture healing and effective parts of split.To study the chemical characteristics of each component elution HPLC spectrum,examine split components of different behavior and the characteristics of ultraviolet absorption peak combined with monomer compounds identified.The results showed that the water elution group is divided into cycloalkene ether terpenoids composition;The group is divided into composition of lignans by methanol.By HPLC feature maps and UPLC QTOF-MS technique application of principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)mathematical statistical methods,such as chemical composition b minutes whether cross validation,the results show that split the iridoid constituents and wooden fat element components are crossed well.By HPLC method,combined with traditional Chinese medicine(TCM)chromatographic fingerprint similarity evaluation system,evaluation of the two types of components of chromatographic fingerprint,and analyze the 10 batches of samples,the results show that the split component preparation process stable and feasible,and has good repeatability.2.Pharmacological activity research of Sambzucus williamsii H.efficacy material basis at all levels.Using rat femur fracture model,through observation of x-rays and blood biochemical index of examining the determination of Sambucus williamsii H.effective parts and its resolution of the chemical components of the therapeutic effect of fracture healing in different periods.The results show that the X-ray observations show that the elder effective parts and iridoid constituents of high,middle dose group prompted the home rats fold zone of fracture healing.Sambucus williamsii H.effective parts and iridoid constituents of high,middle dose group can make the fracture of rat serum ALP,BGP,serum P content increased significantly,serum Ca content decreased obviously.With high dose group of strength is the best.Using the rat model of fracture in the 8 weeks:the hemorheology indexes of whole blood viscosity,plasma viscosity,erythrocyte aggregation index to test.The results showed that the blood rheology basic changes within the normal reference value,that surgery was not on experimental rat blood clots;Compared with model group Sambucus williamsii H.effective parts and wooden fat element groups have significant differences,with promoting blood circulation to remove blood stasis.Adopted the inflammation caused by acetic acid seepage model and rat foot swelling model to investigate the elder and its effective parts cycloalkene ether terpenoids and anti-inflammatory effects of lignans components.Inflammatory exudation in mice model of effective parts and wooden fat element in high dose group compared with model group showed significant anti-inflammatory effect and dose dependent.Effective parts in the model rats foot swelling,iridoid,wooden fat element high dose group showed significant anti-inflammatory effects,but the timing of the work is not the same.Can preliminary thought lignans components is Sambucus williamsii H.anti-inflammatory effects of the main effective components,but may be related to cycloalkene ether terpenoids composition exist mutual synergy effect.With traditional Chinese medicine composition,design of the multi-channel,multi-level and is in line for the characteristics of targets.This experiment for the elder anti-inflammatory mechanism research has laid a theoretical basis.Based on research results,this study completed the docking elder effective parts of chemical separation and verify the split cycloalkene ether terpenoids composition and lignans components are crossed well.Sambucus williamsii H.effective components in promoting fracture healing,promoting blood circulation to remove blood stasis has good effect,anti-inflammatory,in order to further explore its mechanism of action of promoting fracture healing laid the foundation experiment.