The Protective Effects Of Dietary Low Methoxyl Pectin On Acute Pancreatitis And It's Associtated Intestinal Barrier Injury In Mice

Acute pancreatitis(AP)is a common clinical acute abdominal disease.The incidence of AP continued to rise in recent decades.But there is not effective treatment on AP so far.The intestinal injury associated with AP will aggravate the condition retroactively.This study investigates whether the low-methoxyl pectin(LMP)isolated from lemon could attenuate AP and associated intestinal injury.Experimental AP was induced in BALB/c mice by caerulien(CAE)hyperstimulation.BALB/c female mice were randomly divided into three groups: control group(Con group),acute pancreatitis model group(CAE group),low-methoxyl pectin diet group(LMP group).LMP group was pre-fed with 5% LMP supplemented forage 3 days before AP induction.Humane killing was 1 h after the last caerulein injection.We gathered the samples such as serum,pancreas,colons and ileums quickly.We determined serum amylase,lipase and pancreatic MPO by commercial kits.And we determined cytokines by ELISA.The expression of ZO-1,Occludin,DEFB1 and CRAMP were determined by real-time fluorescent quantitative PCR.Histology of pancreas and colons were evaluated by H&E staining.We found that LMP supplementation attenuated the severity of AP as evidenced by reduced serum amylase and lipase levels,pancreatic edema and myeloperoxidase activity.The protective effect was also confirmed by histological examination of pancreatic damage.LMP suppressed the production of pancreatic proinflammatory cytokines including TNF-?,IL-1?,and IL-6.Moreover,LMP supplementation restored AP-associated disruption of intestinal barrier integrity as evidenced by upregulation of tight junction modulatory proteins occludin,zonula occludens(ZO)-1,antimicrobial peptides ?-defensin-1(DEFB1)and CRAMP as well as increase in SCFAs production.LMP supplemented mice with AP exhibited suppressed intestinal inflammation as shown by decreased ileal and colon cytokine production compared with CAE group.Our results support dietary LMP supplementation as an effective nutritional intervention for AP and associated intestinal injury.The mechanism may be related to inflammation inhibition and protection of intestinal mucosal barrier function.LMP as a safe and healthy soluble dietary fiber,may be developed into AP-related functional foods in order to better prevent or alleviate AP.