The Inhibitory Effects Of Fibroblast Growth Factor Inhibitor BGJ398 On The Proliferation,Adhesion And Invasion Of Renal Cell Carcinoma

Objective The expression of fibroblast growth factor receptor(FGFR)is associated with tumor genesis and metastasis.But the research on the expression of fibroblast growth factor receptor in renal cell carcinoma is rare.To study the expression and function of FGFR in renal cell carcinoma,this research focused on the influence of FGFR inhibitor BGJ398 on the proliferation,adhesion,and invasion of human renal cell carcinoma cells OS-RC-2 cells.So the objective of this research is to explain the influence of inhibition of FGFR on the biological function of renal cell carcinoma and provide new method for targeted therapies of renal cell carcinoma.Methods Human renal cell carcinoma cells OS-RC-2 cells were treated with different concentration of 0,0.1,0.5,1,2,5,10μmol/L BGJ398.The survival of cells was supplied by MTT assay.The viability and cytoskeleton of OS-RC-2 cells was checked by confocal fluorescence microscope.The invasion of OS-RC-2 cells was evaluated by Transwell chamber methods.Results BGJ398 could inhibit the proliferation of OS-RC-2.The result of MTT assay revealed that BGJ398 could suppress the survival of OS-RC-2 by concentration dependant manner.Live/Dead fluorescence staining experiments showed that BGJ398 can significantly inhibit the proliferation and viability of OS-RC-2 cells(P<0.01).Cell adhesion and Transwell chamber results show that BGJ398 could inhibit cytoskeleton remodeling and invasion of OS-RC-2 cells(P<0.01).Conclusion BGJ398 can inhibit the proliferation,viability,and invasion of human renal cell carcinoma cells.Hence,inhibition of FGFR could be the therapeutic targets of renal cell carcinoma.We also provided experimental evidence for potential targeted therapies of FGFR inhibitors.