Objective: The study is to investigate the relationship between tumor necrosis factor receptor-associated factor 6(TRAF6)and dendritic cells(DCs)maturation.Method: The bone marrow cells of DBA/1 mice were induced into immature dendritic cells(iDCs)by rmGM-CSF and rmIL-4,and divided into 3 groups(i.e.,the TRAF6 siRNA-transfected group,the control siRNA-transfected group,and the non-transfected group).The iDCs were transfected with siTRAF6,and then stimulated with Lipopolysaccharides(LPS).The mRNA expression of TRAF6 was detected by qRT-PCR.After stimuli by LPS,the expression of CD80,CD86 and MHC-II was analyzed by flow cytometry,and the pro-inflammatory factors were detected by ELISA.Result: The success rate of building CIA model was 84%.The bone marrow cells were successfully induced into iDCs by co-culture the cytokines rmGM-CSF and rmIL-4.Compared with the control siRNA-transfected group and the non-transfected group,the expression of co-stimulatory molecules in the TRAF6 siRNA-transfected group was decreased by flow cytometry.The pro-inflammatory factors in the TRAF6 siRNA-transfected group was inferior to the control siRNA-transfected group and the non-transfected group by ELISA.Conclusion: The mechanism of DCs maturation might be correlated with TRAF6. |