The Role Of PI3K-AKT Signaling Pathway In The Proliferation Of Human Lens Epithelial Cell Which Promoted By Platelet-Derived Growth Factor

Objective: Posterior capsule opacification is the most common complications after cataract surgery and lens trauma.The proliferation of residual lens eithelial cells after cataract surgery and lens trauma plays a very important role in the pathogenesisof posterior capsule opacification.The mechanism of the proliferation of residual lens eithelial cells has not been fully clarified.Platelet-derived growth factor and LY294002 which is one of the PI3 K signal pathway blocher were added to human lens epithelial strain SRA01/04 cells respectively or at the same time.The purpose of our study was to investigate the role of PI3K-AKT signaling pathway in the proliferation of human lens epithelial which promoted by platelet-derived growth factor.Methods : Human LECs strain SRA01/04 cells were culture and passaged.Different concentration of PDGF(0ng/ml,0.1ng/ml,1.0ng/ml,10ng/ml,100ng/ml),LY294002(0?M,5?M,10?M,20?M,40?M)and PDGF(10ng/ml)+LY-294002(0?M,5?M,10?M,20?M,40?M)were added into the medium respectively or at the same time for 24 hours.The proliferation rate or inhibition rate of the cells was evaluated by MTT;then select the drug concentration of different group accroding to the result of MTT,and our study was divided into four groups: blank group,PDGF group(the concentration of PDGF was 10ng/ml),LY294002 group(the concentration of LY294002 was 40?M),PDGF+LY294002 group(the concentration of PDGF and LY294002 were 10ng/ml and 40?M).The lens eithelial cells of different groups were cultured for 24 hours.The cell numbers and morphology was obsered and recorded undered inverted microscope;The protein expression level of AKT and p-AKT for different groups was evaluated using Werstern blot.Results: 1?The proliferation rate of human lens epithelial cells strain SRA01/04 cells was positively correlated with the concentration of PDGF(P<0.001).2?The inhibition rate of human lens epithelial with strain SRA01/04 cells proliferation was positively correlated with the concentration of LY294002(P<0.001).3?The proliferation of human lens epithelial cells strain SRA01/04 cells which effected by PDGF was negatively correlated with the concentration of LY284002(P<0.001).4?There was no significant difference in AKT expression when PDGF and LY294002 was added to human lens epithelial cells strain SRA01/04 cells respectively or at the same time(P=0.066).5?The expression of p-AKT in hunman lens epithelial cells strain SRA01/04 cells increased when PDGF was added alone,the difference was statistically significant(P<0.001).6? The expression of p-AKT in hunman lens epithelial cells strain SRA01/04 cells decreased when LY294002 was added alone,the difference was statistically significant(P<0.001).7?The expression of p-AKT in hunman lens epithelial cells strain SRA01/04 cells was more when PDGF and LY294002 were added at the same time than that of LY294002 added alone,and less than that of PDGF added alone,the difference was statistically significant(P<0.001).Conclusions: 1?PDGF was found to promote the proliferation of human lens epithelial cells.2?LY294002 was found to inhibit the proliferation of human lens epithelial cells.3?LY294002 was found to inhibite the proliferation of human lens epithelial cells which induced by PDGF.4?PDGF was found to promote the proliferation of human lens epithelial cells,which depended on the PI3K/AKT pathway.