Molecular Pathological Research On Prediction Risk Of PTC By Ultrasonography

Objective: The purpose of this study is to discuss the value of ultrasonographic features predict the risk of papillary thyroid carcinoma via combine with molecular pathological research of long non-coding RNA HOTAIR in papillary thyroid carcinoma.Methods: 1.The expression of HOTAIR and clinical information concerning PTC were downloaded from TCGA and analyzed the relationship between expression of HOTAIR and clinicopathologic parameters.2.Summarized the ultrasonographic features of 34 PTC patients,such as number,size,margin,shape,echogenicity,calcifications.3.PTC tissues and non-cancer tissues were collected for RT-qPCR to evaluate HOTAIR expression.4.Analyzed the relationship between ultrasonographic features and expression of HOTAIR.Results: The TCGA database statistics demonstrated that the expression of HOTAIR was significantly increased in PTC tissues compared with non-cancerous tissues(P<0.05).The high expression of HOTAIR was associated with faster tumor growth(P=0.032),lymph node metastasis(P=0.009)and advanced TNM staging(P=0.038),moreover,the overall survival in higher expression patients was significantly shorter than PTC patients with lowerHOTAIR expression(P=0.03).Simultaneously,most of the lesions showed ill-defined(91.2%),taller-than-wide(76.5%),hypo-echoic(94.1%),micro-calcificat ion(67.6%).And,the expression level of the cases with RT-qPCR was consistent with the results of TCGA database,the expression of HOTAIR was up-regulated in papillary thyroid carcinoma tissues(P<0.05).Futhermore,the taller-than-wide in PTC patients was associated with over-expression of HOTAIR.Conclusions: HOTAIR may be involved in papillary thyroid carcinogenesis,the taller than wide in PTC patients was associated with over-expression of HOTAIR,and may be risk factor for preoperative risk assessment of PTC.