| Objective To study the polymorphism of cholesterol ester transfer protein(CETP)gene C>T/In9 in GuangXi area and to study the relationship between the polymorphism and glycometabolism, subclinical atherosclerosis(SA)in type 2 diabetic mellitus(T2DM).Methods All the cases from Guangxi Zhuang Autonomous Region long-term ( > 10 years) unrelated individuals. 83 cases of T2DM , according to 1999 WHO recommended standards of diagnosis and classification of diabetes mellitus, selected in December 2012 to June 2014 in the first affiliated hospital of Guangxi Medical University Endocrinology and metabolism section inpatients with type 2 diabetes, male 51 cases, female 32 cases, age (55.9±9.9) years old.Normal control group: 68 cases selected from our hospital physical examination center health examination population over the same period, ages (52.3±11.4)years old, male 42 cases, female 26 cases, the fasting and postprandial blood glucose excluding diabetes. CETP genotypes of 68 controls subjects and 83 type 2 diabetic patients were analyzed by means of polymerase chain reaction and restricted fragment length polymorphism(RFLP). Height, weight, blood pressure were measured by the same specialized persons. Subjects fasted 12 hours and the next morning their venous blood samples were took. Triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), load lipoprotein A(ApoA), apolipoprotein B(apoB) were detected in Hitachi 7600 automatic biochemical analyzer. The T2DM cases' IMT of bilateral carotid artery and lower extremity femoral artery were tested by the ultrasound imaging professionals. Genotype and allele frequency, clinical parameters and biochemical indexes were compared between T2DM group and control group, SA group and non SA group. The data were analyzed by SPSS 16 software, calculating two groups of genotype and allele frequencies by gene counting method; measurement data with (x ± s), using two independent sample t test. Skewed data by [M (QR)] said, for the rank sum test.The genotype and allele frequency distribution for 2 test, Hardy-Weinberg balance analysis. Multivariate Logistic regression analysis was to analysis the risk factors contributing to T2DM subclinical atherosclerosis.Results: 1. The clinical data and biochemical indicators According to the diagnosis of diabetes recommended by WHO in 1999 and classificationschemes,T2DM group for the number 83, the control group number is 68. The two groups were compared between BMI, TC, sex, TG, LDL-C, the difference was not statistically significant. The two group of age (55.9 + 9.9 VS 52.3 + 11.4,P=0.040), HDL-C (1.37 + 0.31 VS 1.59 + 0.39, P=0.000), the difference was statistically significant (P < 0.05). In the T2DM , SA group (46 people) and non SA group (37 people) compared, No statistical sign:ificance in two sub groups BMI, duration of diabetes ,TC , TG, HDL-C, LDL-C (P>0.05). Age (60.35 + 9.13 VS 50.38 + 7.93, P=0.000), the prevalence rate of hypertension (50% VS 24.3%,P=0.017), the difference was statistically significant (P < 0.05). In patients with T2DM, CETP C>T/In9 3 genotypes (TT, CC, CT) had no statistical significance in TC, TG, HDL-C, LDL-C difference (P > 0.05).2.Hardy-Weinberg equilibrium The distribution of rs289714 genotype frequencies in the control group and T2DM group were consistent with the hardy Weinberg equilibrium (control group: X 2=0.644, P=0.419; T2DM group: X 2=0.306, P=0.580), distribution of each genotype is representative of the population.3. CETP gene rs289714 genotype and allele frequency distribution. There were no significant differences (P > 0.05) between the T2DM group and the control group of the genotypes and allele frequency distribution. In the T2DM ,CT, TT , CC sub genotype distribution ,SA group were 67.4%, 10.9%, 21.7%,non SA subgroups were 45.9%, 2.7%, 51.4%, the difference was statistically significant (X2=8.367, P=0.014). SA subgroup and non SA subgroups T, C allele frequency, the difference was not statistically significant (P>0.05).4. Multivariate Logistic regression analysis T2DM with subclinical atherosclerosis or not as dependent variable and rs289714 genotype (1-TT genotype, 2-CC genotype, 3-CT gene type) (Digital is assignment), age and hypertension as independent variables. Results show that rs289714 genotype, age had significant correlation with diabetic SA [or (95% CI) were 0.560 (0.317,0.989), 1.126 (1.054 and 1.203), P< 0.05].Conclusions: In GuangXi area ,the CETP C>T/In9 genotype in T2DM group and control group ,the difference was not statistically significant ,CETP C>T/In9 genetic polymorphism may have no definite relationship with glycometabolism. CETP C>T/In9 genotype distribution different in T2DM SA and non S A groups, it may be related to the subclinical atherosclerosis in T2DM.The TT may be the harmful genotypic, CETP C>T/In9 genetic polymorphism may be molecular genetic basis for subclinical atherosclerosis in T2DM . In the T2DM ,SA group and non SA group, age and hypertension prevalence rate had a significant difference .Age , hypertension may be highly correlate with atherosclerosis. |
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