The Level And Diagnostic Value Of Interleukin-35 In Tuberculosis Pleural Effusion

Objective: The purpose of the present research was to detect the concentrations of interleukin-35(IL-35) in the pleural effusion .To investigate the diagnostic accuracy of adenosine delaminate (ADA),interleukin-35(IL-35),interferon-y (IFN-y)in the pleural effusion with various etiologies.Methods: One hundred and seventy-six inpatients suffering pleural effusion were included in the study, with 86 cases in tuberculous pleural effusion group and 60 cases in malignant pleural effusion group, 10 cases in bacterial pleural effusion,20 cases in transudative pleural effusion. The levels of IFN-y and IL-35 in pleural effusions were determined by sandwich enzyme-linked immunosorbent assays, the serum levels of IFN-γ and IL-35 were also detected. The detection levels of ADA were completed by our hospital. The data was analyzed with SPSS 19.0 software.Results: The concentrations of IL-35,IFN-γ and ADA in TPE were significantly higher in other pleural effusion. The concentrations of IFN-y and IL-35 in tuberculous pleural effusion were significantly higher than that of peripheral blood. There was no statistical significance in serum concentrations of IL-35 and IFN-y in each group. Analysis result of ROC: The cut-off value of pleural ADA, IFN-y and IL-35 for the diagnosis of TPE were 22.65U/L,206.4pg/ml and 21.17U/ml,respectively .The sensitivity of ADA, IFN-y and IL-35 in pleural effusion were 93.3, 90.7 and 52.3%, respectively, and the corresponding specificity were 93.3,98.9 and 82.2%, respectively. Combinations of IL-35, IFN-γ and ADA yielded the highest diagnostic value. The sensitivity,specificity and Youden index were 98.8% ,95.65,94.4%.Conclusion: The levels of IFN-y and IL-35 in tuberculous pleural effusion are significantly higher than that of the corresponding serum, it suggest that IFN-y and IL-35 secretion of water release are pleural stress chamber local immune response results. The diagnostic value of IL-35 is finity. Area under the ROC curve shows that the highest diagnostic value is IFN-y, followed by ADA.The joint determination of multiple indices, including ADA,IFN-γ and IL-35 has great practical value in the diagnostic of TPE.