Quantitative Peptidomic Analysis Of Cartilage Tissue

Background:Peptidomic is an endogenous peptide produced Intracellular and extracellular.The peptides are constantly active in vivo or produced by proteolytic processing and are involved in a series of physiological functions.Recently,the importance of endogenous peptides in physiology and pathology has become increasingly recognized.With the globalization process of whole genome analysis,the knowledge of gene sequence,protein and peptide expression analysis is greatly increased.In view of the extensive research in the field of peptide,based on the mass spectrometry of peptidomic,focusing on the functional peptide of cartilage development.Objective:In this study,the differential expression of ipslips epiphyseal cartilage peptide in children and infant was quantitatively analyzed by liquid chromatography-tandem mass spectrometry,and the active small molecule peptide related to cartilage development was found in order to further study the physiological function of cartilage tissue.Methods:14 cases of anterior superior iliac spine cartilage were divided into two groups:8 cases of children group(6-8 years)and 6 cases of infant group(less than 3 years old).The extracted cartilage peptides were identified by liquid chromatography-tandem mass spectrometry and the abundance of cartilage peptides in different age groups was compared.Results:By analyzing the cartilage peptides of different age groups,a total of 5027 endogenous peptides were identified and decomposed from 1869 macromolecular proteins.Of which there are 588 differential peptides,derived from 428 kinds of macromolecular proteins.There were high expression of 68 peptides in the children group(more than 3.5 times),high expression of 9 peptides in the infant group(more than 3.5 times),and all high expression of 77 peptides were statistically significant(P<0.05).Conclusion:We use a highly sensitive peptidomic that allows us to identify small molecules of active peptides in normal children's epiphyseal cartilage tissue.These active cartilage peptides may reflect physiological functions of the cartilage tissue development process,but also give us a new perspective to understand the cell differentiation,immune regulation,neurotransmitter regulation,tumor lesions and so on.