Effect Of Runzao Jiedu Tongluo Method On Expression Of M3R In Blood And Submandibular Gland Of SS Mouse

Objective To study the effect of Runzao Jiedu Tongluo method on expression of M3R in of M3R in blood and submandibular gland of SS Mouse,and to explore the therapeutic effect and mechanism of Jiedu Tongluo Shengjin Decoction.Methods Forty-seven BALB/c mouse were randomly divided into making model group(n=37)and normal control group(n=10).Submandibular gland of BALB/c mouse were made into antigen to molding.Mouse of making model group was induced by intradermal injection of antigen in the backs in 5 sites to establish SS model.The succeeded SS mouse were randomly divided into model control group(n=10),TCM group(n=10)and HCQ group(n=10).Mouse of TCM group were given Jiedu Tongluo Shengjin Decoction by stomach rearing according to 11.4g/kg·d.Mouse of model control group were given equivalent Physiological saline.Mouse of HCQ group were given HCQ liquor according to 0.06g/kg·d in the same way.Normal feeding of normal group.Vfter treatment 30 days,spleen?submandibular gland and peripheral blood were collected.Using HE staining method to examine run situation of lymphocyte in submandibular gland of mouse,using ELISA method to determine standard of M3R in peripheral blood of mouse,and using immunohistochemical to determine expression of M3R in submandibular gland of mouse.Results ?Before treatment,compared with normal group,the saliva volume of model group,TCM group and HCQ group decreased,the difference had statistical significance(P<0.05);There was no statistical significance between model group,TCM group and HCQ group(P>0.05).On the thirtieth day of treatment,compared with normal group,the saliva volume of model group decreased,the difference had statistical significance(P<0.05);Group compared with model,the saliva volume of TCM group and HCQ group increased,the difference had statistical significance((P<0.05);There was no statistical significance between TCM group and HCQ group(P>0.05).Compared with own group before treatment,after treatment,the saliva volume of TCM group and HCQ group increased,the difference had statistical significance((P<0.05).? Compared with normal group,the expression of M3R in peripheral blood of model group mouse increased,the difference had statistical significance(P<0.05).The expression of M3R reduced in TCM group and HCQ group compared with model group,the difference had statistical significance(P<0.05).There was no statistical significance between TCM group and HCQ group(P>0.05).? Compared with normal group,the expression of M3R in submandibular gland of model group mouse increased,the difference had statistical significance(P<0.01).The expression of M3R reduced significantly in TCM group and HCQ group compared with model group,the difference had statistical significance(P<0.05).There was no statistical significance between TCM group and HCQ group(P>0.05).?Compared with normal group,run situation of lymphocyte in submandibular gland of mouse of model group mouse increased,the difference had statistical significance(P<0.01).Run situation of lymphocyte in submandibular gland of mouse reduced in TCM group and HCQ group compared with model group,the difference had statistical significance(P<0.05).There was no statistical significance between TCM group and HCQ group(P>0.05).? There was no statistical significance between any group in spleen index and Submandibular gland index(P>0.05).Conclusion The expression levels of M3R in peripheral blood and submandibular gland of SS mouse increase.Traditional Chinese medicine of dryness toxin and dredging collaterals method may by reducing the M3R expression,thus inhibiting the run situation of lymphocyte of SS mouse.