Studies On The Chemical Constituents Of Xylaria Striata And Their Bioactivities

Xylaria striata(Pat.1887)belongs to the genus Ascomycetes and mainly grows on decaying bark or tree roots lived.Except for a long time study on Xylaria striata from our group,there are no reports of Xylaria striata.The activity test showed that Xylaria striata fruiting bodies powder was non-toxic and had a good effect of improving sleep.In order to elucidate the sleep promoting components of this fungus,the chemical constituents were systematically isolated and purified.With the guide of sleep promoting activity,the chemical constituents of Xylaria striata were isolated and purified.The pentobarbital sodium-induced sleep experiment was carried out as the activity guide,and fruiting bodies of Xylaria striata was used as raw materials.Thirteen compounds were isolated from the ethanol extracts of the fungi by column chromatography techniques,including two new and eleven known compounds.Some compounds were tested on improving sleep,anti-tumor and anti-nematode bioactivities,and the aim was to lay a foundation for development of the fungi in related field.(1)The safety of Xylaria striata and its active ingredients with improving sleep were studied.The oral safety was evaluated by acute toxicity test,and the results showed that the mice received Xylaria striata fruiting body powder(2000 mg/kg)had normal physiological indexes and no signs of disease in organs.The active ingredient with sleep improving activity was tracked by the pentobarbital sodium-induced sleep experiments.The final result showed that the active fraction of the fungi was chloroform extract and xylastriamide A and ergosterol from the extract were activity ingredients with improving sleep.(2)Study on chemical constituents of ethanol extraction from Xylaria striata fruiting body.In this paper,the chemical constituents of Xylaria striata were studied systematically for the first time.Thirteen compounds were isolated from the fungi fruiting bodies by recrystallization and various chromatographic techniques.The compounds were identified as: xylastriamide A(1),xylastriasan A(2),cytochalasin D(3),19,20-epoxycytochalasin Q(4),cytochalasin Q(5),19,20-epoxycytochalasin D(6),19,20-epoxycytochalasin C(7),trans-p-hydroxyl cinnamic acid ethyl ester(8),indole-3-carboxaldehyde(9),xanthosine(10),uracil(11),uridine(12),and ergosterol(13).Compounds 1~12 were isolated from the fungi for the first time,and compounds 1 and 2 are new compounds.(3)Sleep improving and anti-tumor and anti-nematode activities of some compounds were tested.Compounds 1~7 were performed by pentobarbital sodium-induced sleep experiments in mice.The results showed that compound 1 can significantly promote sleep in mice.The result of cytotoxic activity in vitro showed that compounds 1 and 9(100 and 40 ?mol/L,respectively)exhibited cytotoxic activity against HCT116,B16 and U937 cell lines by MTT test,and the inhibition rate is more than 50%.Compound 1 possessed moderate anti-nematode activity with IC50 values of 103.54 ?g /mL by using the anti-nematode experiment.