Association Of MTHFR And ABCB1 Genetic Polymorphisms With Methotrexate Adverse Reaction In Childhood Acute Lymphoblastic Leukemia

Objective: To determine the association of methylenetetrahydrofolate reductase(MTHFR)gene A1298 C and C677 T and ABCB1 polymorphisms with methotrexate adverse reaction in childhood acute lymphoblastic leukemia by high-dose methotrexate(HD-MTX).Methods: We collecte the peripheral blood samples of 43 children with ALL accepting the treatment of HD-MTX from February of 2015 to December of 2016.Genotype of MTHFR was detected by PCR-chip hybridization technology.Toxicities of HD-MTX are recorded.Results: The probability of mucosititis and myelosuppression is higher in carriers of MTHFR 677CT+TT genotype as compared with patients with 677 CC genotype(P<0.05).The probability of gastrointestional reactions is higher in carriers of MTHFR 1298 AA genotype as compared with patients with 1298 AC+CC genotype(P<0.05).The probability of myelosuppression is higher in carriers of ABCB1 3435CT+TT genotype as compared with patients with 3435 CC genotype(P<0.05).Conclusions: It is concluded that MTHFR and ABCB1 gene polymorphisms associate with the toxicity of HDMTX chemotherapy in chilidhood acute lymphocytic leukemia.