The Expression And Clinical Significance Of CUGBP-1 And CyclinD1 Proteins In Gastric Adenocarcinoma

Objective:1.Compare the expression of CUGBP-1 and CyclinDl proteins in cancer tissues and normal tissues on the clinical level.2.Explore their clinical significance through analyzing the relationship between proteins' expression and clinical pathological parameters.3.Test the possible correlation between them and their mechanism of action based on the previous experimental result.Method:1.67 cases of gastric cancer tissues were collected from the Second Hospital of Jilin University with detailed clinicopathological parameters in October 2012 to October 2016;Immunohistochemical SP method was used to detect the expression level of CUGBP-1 and then combining with some clinical pathological parameters to find its clinical significance.2.26 cases of gastric cancer tissues and its adjacent non-cancerous tissues were collected after operation in Oct.of 2014 to Oct.of 2016;Western blot assay was used to detect the expression level of both proteins.Reverse transcription-polymerase chain reaction was used to detect the expression level of mRNA in the meantime.Their differences were analyzed by special software to confirm that it is meaningful or not.Result:1.The result of Western-blot showed the expression level of CUGBP-1 protein in gastric cancer tissues(0.652±0.2252)was higher than its adjacent non-cancerous tissues(0.442±0.2190),the difference was statistically significant(p<0.05).The expression level of CyclinDl protein in gastric cancer tissues(1.165±0.0856)was higher than its adjacent non-cancerous tissues(0.581±0.1632),the difference was statistically significant(p<0.05).2.The result of RT-PCR showed that the expression of mRNA in gastric tissues(4.485±0.6501,2.342±0.2007)was obvious higher than its adjacent non-cancerous tissues(2.457±0.7684,1.654±0.2296)and the difference was statistically significant(P<0.05).3.The result of IHC showed that the CUGBP-1 protein expressed in gastric cancer tissues and adjacent non-cancerous tissues and mainly located in the cell nucleus.The rate of its expression in gastric cancer tissues(80.60%)was considerably higher than that in adjacent non-cancerous tissues(47.40%)(?2 =12.423,p<0.05).4.The high expression of CUGBP-1 protein in gastric cancer was associated with its lymphatic metastasis,pTNM stage(P<0.05).But there was no specific association with patients' gender,age and tumor size and the grade of differentiation(P>0.05).Conclusion:1.The level of CUGBP-l's expression is associated with lymphatic metastasis,pTNM stage and grade of differentiation and it may provides us with a possible useful indicator for the malignant of gastric carcinoma and its metastasis potential.2.Two proteins both have the higher expression in gastric cancer tissues and combining our previous experimental result(the silencing of CUGBP-1 gene can suppress the expression of CyclinDl and then suppressed the proliferation of gastric cancer cell line MGC-803)with their expression synchronism at the level of protein,we may find a new action target to treat gastric cancer by biotherapy in the future.