| ObjectiveBy feeding high sugar and fat diet,combining the method of intraperitoneal injection of STZ,the Wistar rats were induced into early type 2 diabetes mellitus model,gavage for traditional Chinese medicine Yishenhuoxuefang(YSHXF)particle,then,daily for 8 weeks,to observe the protective effect on T2 DM early diabetic nephropathy in rats.Methods90 Wistar rats,clean healthy male,adaptive feed for a week,10 rats were randomly selected as control group,the rest 80 rats feed high sugar and fat a month,and were intraperitoneal injected STZ 25mg/kg as model group rats.The blood sugar value of 7.8 mmol/L or greater tendency after test 72 h fasting blood glucose,for building successful model rats,and throw out not as a model and death in the rats.The successful model rats were divided into five groups randomly: model group,Benazepril Hydrochloride(1mg/kg)group,YSHXF particle 2.0 g/kg group,YSHXF particle 1.0 g/kg group,YSHXF particle 0.5 g/kg group.All the animals were gavage administration for medicine,daily for 8 weeks.The general situation,weekly drinking water and eating,weekly weight,biweekly to determine the tail vein fasting blood sugar levels were record.One day before the end of the experiment rats were fasted water collected 24 h urine,determination of urine creatinine(UREA),?2-microglobulin(?2-MG).In the surgery day,intraperitoneal injection of 10% chloral hydrate 30mg/kg anesthesia,abdominal aorta in blood,the determination of cholesterol(CHO),triglycerides(TG),glutathione peroxidase(GSH-PX),superoxide dismutase(SOD),maleic dialdehyde(MDA).Collected rats left kidney,PAS staining to observe rat kidney pathological changes,and the expression of immunohistochemical method in TGF-?,Smad2/3,Smad7 protein.ResultsCompared with control group,model group rats: 1)depression,dark color,unresponsive,appear obvious symptoms: more drinks,food,urine,loss of weight(P<0.01 or P<0.05);2)blood glucose were significantly higher in 0,2,4,6,8 week(P<0.01);3)urine UREA,?2-MG content were significantly increased(P<0.01);4)serum CHO,TG content were significantly higher(P<0.01);5)serum GSH-PX,SOD of model group activity were decreased,MDA content was significantly increased(P<0.01);6)kidney tissue pathological observation: kidney basement membrane thickening,glomerular volume increase;7)kidney immunohistochemical staining observation: TGF-? and Smad2/3 gray values decreased,Smad7 gray value increased,indicating TGF-? and Smad2/3 protein higher expression,Smad7 protein lower expression(P<0.01);Compared with model group: 1)benazepril group,YSHXF particle 2.0,1.0g/kg group has improved mental state,color,the increase of food quantity,and water quantity,and weight loss of the phenomenon is also a marked change(P<0.01 or P<0.05);2)benazepril group and YSHXF particle 2.0,1.0g/kg group can significantly lower blood glucose levels in rats after treatment(P<0.01 or P<0.05);3)benazepril group and YSHXF particle 2.0,1.0,0.5g/kg group can significantly reduce rats urine UREA,?2-MG content(P<0.01 or P<0.05);4)benazepril group and YSHXF particle 2.0,1.0,0.5g/kg could significantly reduce CHO,TG content in serum of rats(P<0.01 or P<0.05);5)benazepril group and YSHXF particle 2.0,1.0g/kg group could increase serum GSH-PX,SOD activity,and reduce MDA content(P<0.01 or P<0.05);6)kidney tissue pathological observation: benazepril group and YSHXF particle 2.0,1.0g/kg group kidney basement membrane thickening,glomerular volume increase phenomenon have seen obviously improved;7)kidney immunohistochemical staining observation: benazepril group and YSHXF particle 2.0,1.0,0.5g/kg group TGF-?,Smad2/3 gray values increased,Smad7 gray value decreased,indicating TGF-??Smad2/3 protein expression decreased,Smad7 protein expression increased(P<0.01 or P<0.05).YSHXF 2.0,1.0g/kg have not marked change.ConclusionYSHXF particle can significantly improve the general symptoms of T2 DM early diabetes nephropathy,regulate glucose and lipid metabolism disorder,regulate the TGF-? signal path TGF-??Smad2/3?Smad7 protein expression,protect renal function. |
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