Preparation Of Nano-hydroxyapatite Coating On Tixos Surface And Its Effect On Adhesion And Proliferation Of Schwann Cells

Background:With the development of fundamental research on implant mechanism and the progress of materials science,implant denture has been getting more and more used in clinics.At present,osseointergrated implant denture have been the primary restoration of dentition defect and edentulous jaw,which can not be replaced in clinical treatment.Implant is more sensitive than natural teeth because there is lack of periodontal ligaments around them and the physiologic mobility is limited.That means when suffered from excessive force,the peri-implant bone will be absorbed,eventually leading to the failure of implantation.Therefore,We should pay more attention to regeneration of peri-implant nerve after implant implantation.With regard to implants,its surface features have been a hot topic in the field of discipline.Titanium(alloy)hydroxyapatite composites material not only have the characteristics of small density,low toxicity,excellent biocompatibility and strong corrosion resistance,such as titanium(alloy),but also have high biocompatibility,bone conduction and biological activity of hydroxyapatite to meet the biological and mechanical needs of repair and replacement of bone and teeth.the surface of hydroxyapatite implants can promote the biological behavior and conduction properties of nerve cells.It was popular and became an important clinical biomaterial products.Obiective and methods:Part 1: Preparation of nano-hydroxyapatite coating on Tixos surface.The hydroxyapatite coating with different deposition time on the surface of Tixos by electrochemical deposition.The experiment was divided into the five groups according to the chronopotentiometry.The effect of deposition time on the properties of the electrodeposited coating was studied to determine the optimal electrodeposition parameters.Part 2: Effects of modified Tixos on early proliferation and adhesion of Schwann cellsTo select the surface of the coating which was favorable for cell adhesion and proliferation by cell experiment,to provide experimental basis for improving the surface design of new Tixos.Results:1.All the deposition coatings in different electrochemical deposition time are the hydroxyapatite.The coating thickness increases with the deposition time.The coating thickness is 0.8 to 3.5?m,when the electrochemical deposition time is 40 to 80 minutes;20min group,the cystal on the surface of the material was scattered clutter distribution and arranged sparse.The 40/60/80 min groups,We can see the nano-sized crystals of Tixos surface are porous,uniform,well-ordered and latticed.With the increase of deposition time,the grain arrangement is more intensive and the size is about 80-100 nm,evenly distributed;2.The result of the control group Tixos surface is hydrophobic,and Tixos surfaces with nano-hydroxyapatite coating on it are hydrophilic.The longer the deposition time,the smaller the contact angle.40/60/80 min group showed obvious hydrophilicity;3.The schwann cells morphology and diameter size on the modified Tixos surface are better than the corresponding titanium coating group surface,which spread well and are fused to the coating;4.The 40/60/80 min groups nano-hydroxyapatite coatings on "Tixos" can enhance schwann cells' adhesion and proliferation.Conclusion:1.Electrochemical deposition method can deposit hydroxyapatite on the surface of Tixos,The 40/60/80 min groups,We can see the nano-sized crystals of Tixos surface are porous,uniform,well-ordered and latticed.the grain size is about 80-100 nm and the coating thickness is 0.8 to 3.5 ?m,increasing with the deposition time;2.The Tixos surface modified by nano-hydroxyapatite,40/60/80 min group showed obvious hydrophilicity;3.The 40/60/80 min groups nano-hydroxyapatite coatings on "Tixos" can enhance schwann cells' adhesion and proliferation.