Relationship Between The Expression Of TMEM16A And The Prognosis Of Colorectal Cancer

Purpose:To investigate the relationship between TMEM16A in the colorectal cancer and the prognosis of patients.Methods:1.96 patients with colorectal cancer that were confirmed by pathology after the operation in the First Affiliated Hospital of Dalian Medical University from June 2009 to December 2011 were enrolled and followed-up.All the patients had not gone through chemotherapy or other antitumor therapy,and they also had not other malignant tumors.The patients included 58 males and 38 females,aged 30-80 year-old,with median age of 62.2 years;13 cases with well-differentiated,61 cases with moderately differentiated and 22 cases with the poorly differentiated;lymphnode metastasis in 33 cases,and 63 cases without lymphnode metastasis;6 patients in stage of Dukes A,57 patients in stage of Dukes B and 33 patients in stage of Dukes C,All the patients’ clinical data is integrity.The survival time was from the date of surgery to the date of death or the last follow-up.2.The expression of TMEM16A protein in colorectal cancer was detected by immunohistochemistry in 96 cases.3.The relationship between the expression of TMEM16A protein in colorectal cancer and the clinical features and clinical prognosis were analyzed.The Kaplan-Meier survival curves of high expression group and low expression group of TMEM16A protein were drawn.The difference of survival time between the two groups was compared by Log-rank test.Univariate survival analysis was performed to determine the prognostic factors associated with poor survival of patients after the operation.Multivariate regression analysis was performed to determine whether the high expression of TMEM16A protein was an independent prognostic factor for patients with colorectal cancer.Results:l.Immunohistochemical staining:Among 96 cases,44cases(45.8%)with high expression of TMEM16A protein,and 52 cases(54.2%)with low expression.2.The relationship between TMEM16A protein expression in colorectal cancer and the patients’ clinical pathological parameters:There was no correlation between the TMEM16A protein expression and the gender,age and tumor location,size,degree of differentiation(P>0.05).While the expression of TMEM16A protein was significantly associated with the depth of invasion,lymph node metastasis,Dukes stage(P<0.05).In the group of infiltration≤T2,the high expression rate of TMEM16A protein was 0.00%;while in the group of infiltration>T2,the high expression rate of TMEM16A protein was 48.89%,there was significant difference in the two groups(X~2= 5.415,P = 0.02);In the groups of Dukes stage A and B,the high expression rate of TMEM16A protein was 34.92%,while in the group of Dukes C,the high expression rate of TMEM16A protein was 66.67%,there was significantly difference in the two groups(X~2= 8.791,P=0.003);The high expressions of TMEM16A protein in colorectal cancers with lymph node metastasis(66.67%)were higher than those without lymph node metastasis(34.92%)(X~2= 8.791,P=0.003).3.The relationship between the TMEM16A protein expression and the clinical prognosis:Kaplan-Meier survival curves showed that patients with high-expression of TMEM16A protein had poorer overall survival compared withthose with low-expression level of TMEM16A protein(68.2%vs.92.3%,X~2=9.892,P=0.002).Up-regulation of TMEM16A protein expression can be identified as an independent predictive factor for the poor prognosis in the patients with colorectal cancer.Conclusions1.The expression of TMEM 16A protein in colorectal cancer was associated with tumor invasion,lymph node metastasis and Dukes stage.2.The high expression of TMEM 16A protein in colorectal cancer can be identified as an independent predictive factor for the poor prognosis of patients with colorectal cancer.3.The age,Dukes stage,lymph node metastasis,the expression of TMEM16A protein and the postoperative radiochemotherapy were all associated with the prognosis of patients with colorectal cancer.