| Objective:The present study aimed to investigate the synergistic therapeutic effect of decreaseing cartilage angiogenesis via exposure to crizotinib encapsulated by chitosan microspheres and photo-crosslinked hydrogel,with the goal of evaluating crizotinib as a treatment for osteoarthritis.Methods:First,we developed and evaluated the characteristics of hydrogels and chitosan microspheres.Next,we measured the effect of crizotinib on the cartilage degeneration induced by interleukin-1? in chondrocytes.Crizotinib ameliorated the pathological changes induced by interleukin-1? via its anti-angiogenesis function.In addition,we surgically induced osteoarthritis in mice,which were then injected intra-articularly with crizotinib-loaded biomaterials.Cartilage matrix degradation and expression of vascular endothelial growth factor were evaluated after surgery.Results:Treatment with the combination of crizotinib-loaded biomaterials retarded the progression of surgically induced osteoarthritis.Crizotinib ameliorated cartilage matrix degradation by promoting anti-angiogenesis.Conclusions:Our results demonstrate that the combination of photo-crosslinked hydrogel and crizotinib-loaded chitosan microspheres might represent a promising strategy for osteoarthritis treatment. |
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