Correlational Research Of Relative Leukocyte Telomere Length In Peripheral Blood Of Acute Atherothrombotic Stroke In Patients

Objective:Ischemic Stroke is a set of clinical syndrome of vascular origin,the consequences serious and long.With high incidence,high morbidity,high mortality and high rate of relapse of "four high" characteristics,is one of the leading causes of death and disability in the world,is one of the important killer harm the elderly health and life.This experiment by comparing the atherosclerotic process in the acute cerebral infarction patients with peripheral blood leukocyte telomere length relatively change,try to analyze the mechanism underlying the change of telomere length.Methods:We enrolled 224 patients diagnosed with Acute atherothrombotic strokewho were admitted from the department of neurology,The First People's Hospital of Huainan,Anhui province between June 2015 and August 2016.A total of 616 age-,and gender-and resident area-matched control subjects were recruited from the local community-based inhabitants.The relative telomere length in genomic DNA extracted from circulating leukocyte was measured by real time quantitative polymerase chain reaction.The telomeres was divided into tertile,and logistic regression analysis was used to determine the risk of Acute atherothrombotic stroke and relative telomere length.Results:1.The blood pressure,blood sugar,blood lipids,blood uric acid,smoking,drinking of Acute atherothrombotic strokewere significantly higher than that of control group,the difference was statistically significant,(P<0.05);2.The telomere length ratio was significantlyinversely correlated with age in control subjects(correlation coefficient g =-0.282,P< 0.05)and in patients with acute atherothrombotic stroke(correlation coefficient g =-0.168,P< 0.05);3.we found that individuals in thelowest tertile of telomere length had a significantly higher risk of acute atherothrombotic stroke [multivariate OR,2.33(95%CI,1.42-3.83);P = 0.003] than did those in the highest tertile;4.The multivariate odds ratio [OR](95% confidence interval[CI])was [1.48(1.24-1.76),p<0.001] for acute atherothrombotic stroke in per 1 S.D.decrease in telomere length;5.we conducted analyses stratified byage,gender,BMI,smoking,alcohol intake,hypertension and diabetes and by evaluating interaction terms,There was no statistically significant influence(P>0.05);6.Comparing with the perfect case-control group,we found that individuals in thelowest tertile of telomere length had a significantly higher risk of acute atherothrombotic stroke [multivariate OR,3.60(95%CI,1.77-7.33);p<0.001] than did those in the highest tertile.7.After adjustment for conventional vascular risk factors,we found that those individuals at the lowest tertile of telomeresand also with ahistory of hypertension had a 4.13-fold(95% CI,2.45–7.16;Ptrend<0.001)increased presence of atherothromboticstroke when compared withsubjects at the highest tertile of telomeres and without history of hypertension.Conclusion:1.Peripheral blood leukocyte telomere length relatively increased continuously shorten with age;2.Comparing with the control group,the leukocyte telomere length shortened significantly in acute atherothrombotic stroke.And shorten telomere length was a predicted index foratherothrombotic stroke.