Effect Of Transplantation Of Human Umbilical Cord Blood Mesenchymal Stem Cells On The Expression Of Foxp3 Gene In Peripheral Blood Of Rats With Cerebral Ischemia

Objective To investigate the possible mechanism of human umbilical cord blood mesenchymalstem cells(hUCB-MSCs)on cerebral ischemia injury,Foxp3 mRNA in peripheral blood was observed after hUCB-MSCs transplantation on rats with cerebral ischemia.Methods 100 healthy male SD rats were randomly divided into four groups: sham operation group(n=10),ischemia reperfusion(I/R)group(n=30),saline group(n=30),hUCB-MSCs group(n=30).The expression of Foxp3 mRNA,IL-10 and TNF-alpha was determined by RT-PCR on days 1,7,14,28 after reperfusion in peripheral blood and brain tissue.CD4+CD25+Foxp3Treg cells percentage of CD4+T cells was measured by flow cytometry in peripheral blood.Nerve function defect was scorde by modified Neurological Severity Scores(m NSS).Neuron apoptosis was detected by TUNEL.Results 1)m NSS on days 14,28 in hUCB-MSCs group was significantly lower than that of I/R group and saline group(P< 0.05).2)The number of apoptotic cells in hUCB-MSCs group was significantly lower than that of I/R group and saline group on days 28.(P< 0.05).3)the peripheral blood Foxp3 mRNA expression of I/R group,saline group and hUCB-MSCs group was significantly higher than that of sham operation group on days1,7,14,28(P< 0.01).Foxp3 mRNA of hUCB-MSCs group in the peripheral blood expression was significantly higher than that of the I/R group and saline group on days 1,7.(P< 0.05).4)The expression of IL-10 in hUCB-MSCs group was significantly higher than that in saline group and I/R group on days 7,14.(P< 0.05).The expression of TNF-?was significantly lower in hUCB-MSCs group than that in saline group and I/R group(P< = 0.05)on days 7,14.5)Compared with the sham operation group,CD4+CD25+Foxp3Treg cells ratio of I/R and saline group was significantly increased in ischemia reperfusion injury on days 14,28.(P < 0.05)Compared with the sham operation group,CD4+CD25+ Foxp3 Treg cells ratio of hUCB-MSCs group in ischemic brain damage cells significantly increased on days 7,14,28(P < 0.05);CD4+CD25+Foxp3 Treg cells ratio of I/R group and the saline group was significantly lower than that of hUCB-MSCs group on days 7,14,28.(P < 0.05)Conclusion The mechanism of hUCB-MSCs transplantation in improving ischemic brain damage in rats may be related to the up regulation of Foxp3 mRNA expression in the early stage of inflammatory reaction.