The Efficacy And Toxicity Study On Of GUI QI Sheng XUE Granule In The Treatment Of Aplastic Anemia

bjective: Study on the pharmacodynamics and toxicology of anti-aplastic anemia caused by GUI QI Sheng Xue Granule.Methods : The normal animal and animal disease model,comprehensive evaluation of GUI QI Sheng Xue Granule elevated white blood cell,red blood cell,in order to determine the effect of increased effect of GUI QI Sheng Xue Granule;through the observation of GUI QI Sheng Xue Granule once a litre of blood particles of mice after intragastric administration,toxicity and death of,for clinical reference medication safety;observation of medication for 90 days,180 days after the toxic reaction of animal and animal 30 days after withdrawal of recovery,and the detection indexes,understand the toxicity reaction on the preparation of rats,and provide reference for clinical safe medication.Results:(1)The large dose group of GUI QI Sheng Xue Granule has obvious inhibitory effect on the WBC,RBC,HGB and PLT reduction in mice with aplastic anemia.Two liters of GUI QI Sheng Xue Granule 58.2g crude drug /kg dose group can effectively antagonize the cyclophosphamide induced peripheral white blood cells decreased white blood cells can inhibit radiation induced and thrombocytopenia,can promote bone marrow cells of DNA mice decreased synthesis of white blood cells,promote the formation of mouse spleen nodules;GUI QI Sheng Xue Granule middle dose group can cause a potent antagonist of cytarabine induced decrease of peripheral white blood cells,from benzeneinduced rat peripheral white blood cells and cyclophosphamide reduced peripheral blood cell reduction.(2)GUI QI Sheng Xue Granule 58.2g crude drug /kg dose group can effectively antagonize the cyclophosphamide induced peripheral white blood cells decreased white blood cells can inhibit radiation induced and thrombocytopenia,can promote bone marrow cells of DNA mice decreased synthesis of white blood cells,promote the formation of mouse spleen nodules;GUI QI Sheng Xue Granule,the middle dose group can cause a potent antagonist of cytarabine induced decrease of peripheral white blood cells,from benzene induced rat peripheral white blood cells and cyclophosphamide reduced peripheral blood cell reduction.(3)GUI QI Sheng Xue Granule 58.2g crude drug /kg group of mice hemorrhagic anemia had good inhibitory effect,red blood cells and hemoglobin were significantly increased;reduce the inhibitory effect of Guiqi the dose of blood particles acetylphenyl hydrazine induced mouse peripheral blood erythrocytes and hemoglobin;inhibit the decrease of GUI QI Sheng Xue Granule 29.1g 58.2g/kg raw drug dose of acetylphenylhydrazine induced rat peripheral blood red blood cells and hemoglobin;GUI QI Sheng Xue Granule 29.1g,58.2g crude drug /kg group of rats induced by adenine inhibited the decrease of peripheral red blood cell,the high dose group of hemoglobin decreased or increased role.(4)GUI QI Sheng Xue Granule extract mice gavage once the maximum dose of 104.76 g crude drug /kg,continuous observation for 14 days,animal appearance,behavior,mental state,and its color,size,color,hair,nose,eyes,mouth breathingsecretions were normal,no end of gross anatomy experiment obvious pathological changes.(5)Administration of 90 days and 180 days,GUI QI Sheng Xue Granule of rats feeding behavior,no abnormal administration of rats body weight increased and the value of the control group showed no significant difference(P>0.05);GUI QI Sheng Xue Granule of rats in each group were WBC,RBC,HGB,HCT,MCV,MCH,MCHC,PLT and other blood index is basically normal,compared with the control group was not statistically significant(P>0.05);AST,ALT,biochemical indexes of ALP,CK,TP,Alb,BUN,Crea,GLU,TG,CHOL,TBIL,K,Na,Cl and other blood no GUI QI Sheng Xue Granule of rats abnormal,in addition to individual indicators,most as compared with the control group no significant difference(P>0.05);the autopsy,the organs found no obvious abnormalities,in addition to individual organ coefficient,no significant difference compared with the control group the majority of organ coefficient(P>0.05);and the recovery period of organ There was no significant difference between the control group and the control group(P>0.05);Histopathological examination results reflect: the test groups,some rats had lung interstitial infiltration of inflammatory cells and prostate interstitial infiltration of inflammatory cells,according to the literature,for the rat common spontaneous lesions in the control group,and the high dose group can be seen,the incidence and severity of lesions are basically the same,the two groups,no significant the pathological difference.It can eliminate the drug toxicity caused by pathological changes;parathyroid gland and breast moremissing observations,though there is no exception,but the amount of data is not enough to support the test of the above organs did not produce toxic effect conclusion.In addition to the above,the change of experimental animal drug skin and main organs of heart and liver,spleen,lung,brain,circulatory system,nervous system,digestive system,hematopoietic system,endocrine and reproductive system of male and female animal showed no obvious drug related pathological injury.There was no delayed toxic reaction in the organs of rats in recovery period.Results:It has the effect of anti aplastic anemia,which belongs to no obvious toxic drugs.