| Chinese herbal medicine processing,as one of the characteristics of national medicine,is different from other herbal medicine,and its purpose is"synergistic."The composition of the change in the process of Chinese herbal medicine's processing is very complex,often occurring hydrolysis,binding,decomposition,complex and other reactions.Therefore,the quality of concocted herbs will directly affect the efficacy and safety of the traditional Chinese medicine.At present,the standardization of Chinese medicine and the quality control of processed herbs are still very weak,which leads to the spotty quality of Chinese herbal medicine,and also seriously affects the quality of Chinese medicine.Therefore,it is urgent to study the rapid quality control of the processed medicinal materials and the analysis method of the processing process,and then establish the quality control index of the processed medicinal materials.At present,the research and identification of Chinese herbal medicine are mainly based on the traditional HPLC and HPLC-MS/MS,gas chromatography and its combination technology to determine the chemical composition of traditional Chinese medicine.The method is time-consuming and cannot directly analyze the product and of Low volume,besides,it cannot quickly scan the product and its authenticity identification.This paper will concentrate on the analysis of three kinds of medicinal materials Atractylodes macrocephala,Magnolia officinalis and Cornus officinalis and the feasibility of the desorption corona beam ionization mass spectrometry(DCBI-MS)and Paper spray ionization mass spectrometry(PS-MS).The results show that the combination of ChemPatternTM metrology software can effectively distinguish the processed and raw medicinal materials,and can also track the component variables in the process.The results show that the method can be used as an ultra-fast quality control method.Changes in composition,for the quality of processed herbs are of great significance.The main research contents are as follows:(1)The feasibility of rapid identification of DCBI-MS for processed and unprocessed Atractylodes macrocephala was investigated.At the same time,the transformation of atractylone was tracked in situ.The results could be used to investigate the temperature and the conversion rate.The best processing temperature of Atractylodes macrocephala is120~140?.60 samples of the batch were detected under positive ion mode of the current of 30?A,ion source temperature of 20?and helium flow rate of 1.5 mL/min.The obtained mass spectrometry data was transformed into CDF format and introduced into ChemPatternTM.The results showed that the similarity between non-processed of Atractylodes was 0.90~0.95,and processed in soil of Atractylodes macrocephala was 0.83~0.90,and processed in wheat of Atractylodes macrocephala was at 0.71~0.82.Intuitively dividing the raw white and processed products into three categories can be used as a basis for the identification of Atractylodes macrocephala and its processed products.This proves that this method is also feasible in practical application.(2)The chemical constituents of Cornus officinalis were enriched by PS-MS,and ESI-MS was used to verify the attribution of 5-HMF and7-?-ethoxylated in the eight effective chemical constituents Chimonolide,which can be used as a basis for the identification of processed and non-processed of Cornus officinalis.Finally,the PS-MS method was used to study the changes of the ratio of loganin and morroniside in the processed and non-processed of Cornus officinalis.The results showed that the ratio of Loganin and Morroniside in Cornus officinalis was between 1.1 and 1.2,and the ratio of processed of Cornus officinalis ratio of 0.8 or so,which can be used as the basis of quality control.(3)DCBI-MS was used to analyze non-processed and processed in ginger of Magnolia officinalis.The mass spectrometry MS~2 signal was collected by thickening Magnolol and Honokiol.Isomers were confirmed.The quantitative analysis of Magnolol and Honokiol were determined by high performance liquid chromatography(HPLC).Besides,a new rapid identification method was established by using PS-MS.A total of 30batches of processed products and Magnolia officinalis were tested by paper spray ionization(PS-MS)and converted to CDF format into ChemPatternTM metrology software processing,including similarity,principal component analysis,partial least squares analysis and clustering analysis.The similarity of non-processed is between 0.70 and 0.90,and processed in ginger is between 0.50 and 0.70.The total contribution of PC1,PC2 and PC3 is more than 80%.Partial least squares analysis and cluster analysis can also be analyzed intuitively the division of two types of non-processed and processed in ginger of Magnolia officinalis.Therefore,they can be used as a basis for the identification of non-processed and processed in ginger of Magnolia officinalis. |
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