The Effect Of Rifapentine Sustained-release Microspheres On Rabbit Adipose-derived Stem Cells

Objective : To investigate the dffect of rifapentine sustained-release microspheres with different doses solution on the rabbit adipose-derived stem cells’ growth and proliferation in vitro.Methods : Adipose tissue collected from bilateral inguinal in healthy New Zealand white rabbits was digested,washed inoculation in culture flask.The DMEM liquid was changed once every two days,the growth situation and morphological characteristics of adipose-derived every day.The cell number was counted every day and groth curve was drawn after 10 days.Rifapentine polylactic acid mocrospheres were prepared by using a double-emulsion solvent-extraction technique.The third generation of adipose stem cells were dealt with five different doses of Rifapentine sustained-release microspheres concentration,97.6 、 121.9 、 146.3 、 170.7 、 195.1 ug/m L respectively,then compared the cells growth and proliferation in the five groups by CCK-8 colorimetric method.Results : There is no statistical difference in the effect on growth of cells among five different doses of Rifapentine sustainde-release microspheres concentration(97.6 、121.9 、146.3 、170.7 、195.1 μg/m L)(P>0.05).Conclusion:the influence of Rifapentine microspheres on rabbit-adipose derived stem cell growth and proliferation is neligible.