Effect Of Erythropoietin And Helix B Surface Peptide On Kidney Injury And Inflammatory Response Induced By Urinary-derived Sepsis

Objective:To investigate the effect of erythropoietin(EPO)and helix B surface peptide(HBSP)on kidney injury and inflammatory response induced by urinary-derived sepsis.Methods:Twenty-four New Zealand rabbits were randomly divided into Sham group,Urosepsis(US)group,EPO group and HBSP group.Upper urinary tract obstruction and acute infection was induced to establish sepsis model.Before surgery,12 h,24h,36 h after surgery,blood was taken to examine creatinine(Cr)?blood urea nitrogen(BUN)?alanine aminotransferase(ALT)? aspertate aminotransferase(AST),tumor necrosis factor(TNF)-a and interleukin(IL)-6 are measured by Enzyme-linked immunosorbent assay(ELISA).Kidney?liver and lung tissues were stained with hematoxylin and eosin(H&E)36h after surgery and morphological changes were observed under an optical microscope.Results:At 12 h,24h and 36 h after surgery,levels of Cr?BUN?ALT?AST?TNF-a?IL-6 were all significant elevated in US group compared with the Sham group(P<0.01).When compared with US group,there is no significant decline in EPO treatment group and HBSP treatment group(P>0.05).The histopathological changes were observed under light microscope.Compared with US group,there was no significant difference in the injury scores of kidney,liver and lung tissue in EPO treatment group and HBSP treatment group(P>0.05).Conclusion:EPO and HBSP have no significant protective effect of acute renal injury in this urinary-derived sepsis rabbit model,and did not inhibit the inflammatory response.