Experimental Study On Bei Mue Xiao Effect Of Chinese Herbal Compound On Proliferation And Apoptosis Of FRTL-5 Cells

Objective:Using the research method of cell culture in vitro,Bei Mue observed from the cell level influence elimination FRTL-5 cell proliferation and apoptosis in cultured Chinese compound cells,To explore the mechanism of the effect of Chinese herbal medicine on the thyroid enlargement caused by the principle of "promoting blood circulation,phlegm and trash".Methods:(1)Cell proliferation inhibition assayThe subjects were divided into five groups:normal A:group:normal culture,without any treatment;B:blank serum control group:cultured with 1%normal animal serum medium;C:low-dose group,adding 1%animal serum containing culture medium dose;group D:,cultured with 5%animal serum containing medium;E:large dose group,added to the culture medium 10%containing animal serum.Logarithmic growth phase FRTL-5 cells were seeded in 96 well plates,cultured with different drug concentrations,24h and 48h in the observation of cell proliferation,the absorbance in the wavelength calibration of 450nm enzyme was detected by CCK-8 method(OD)calculation.Mue shellfish growth inhibition rate of elimination of serum containing FRTL-5 cells.(2)Bax,Bcl-2 detection of mRNAThe cells of each group were collected and RNA was extracted.The mRNA expression of Bax and Bcl-2 was detected by real-time fluorescence quantitative PCR.Analysis of different concentrations of shellfish Mue medicated serum inhibition and elimination of Bax cells on the proliferation of FRTL-5 cells in different time by using SPSS 17 software,Bcl-2 mRNA expression data.Results:(1)the concentration of serum Bei Mu E Xiao confirmed shellfish elimination can inhibit the proliferation of FRTL-5 cells was detected by CCK-8 method.(2)Bei Mue medicated serum can make the elimination of thyroid cell apoptosis promoting gene Bax,Bcl-2 expression level of mRNA increased significantly,especially in the large and middle dose group,Chinese medicine group the Bax/Bcl-2 ratio were increased,the difference in dose group significantly(p<0.05).Conclusion:Bei Mue Xiaomedicated serum can inhibit the proliferation of thyroid cells and promote the apoptosis of thyroid cells,can induce the apoptosis gene,suggesting that Beckham Mue elimination Chinese herbal compound in the treatment of thyroid enlargement may inhibit thyroid cell proliferation and promote apoptosis of thyroid cells and realize.