Protective Effects And Mechanism Research Of Ginsenoside Rb-1 On LPS-induced Renal Inijury In Sirs

ObjectiveTo investigate the mechanism of ginsenoside Rb-1 regulating lipopolysaccharide on systemic inflammatory response syndrome involving renal injury,and to provide theoretical and experimental basis for further study of systemic inflammatory response syndrome.Method60 SPF BALB/C mice were randomly divided into blank group(Normal group),model group(LPS),positive drug group(Dex)and ginseng saponin Rb-1 Low,Medium and High dose group(Rb-1 L,M,H group)six groups,each group of 10.The model of SIRS renal injury was established by LPS induction method.After the model 12h,the eyeball took blood and executed the mice.Collect all blood samples and remove the liquid for testing.Record and compare the general status,groups of mice kidney inDex,serum ALT,AST,CREA,BUN,cytokine TNF-α and IL-β level,the pathological and histological changes of kidney and immunohistochemical method to detect kidney NF-κB p65 andp-IxB a predominate the expression of protein levels.Result1.The mouse morphological observation:the mental state and activity of the blank group were normal.In the model group,the mouse was injected with an LPS,which was low in the abdomen.The group and the treatment of each dose group of mice tended to have a smooth breathing,and they were able to keep warm,the skin smooth,the activity and the state of mind were good,and the defecating condition was basically normal.2.The effect of the kidney inDex on mice:the LPS kidney inDex was significantly higher than the blank group,with significant statistical significance.Compared to the LPS group,the Dex group and the treatment of each dose group had significantly reduced renal inDex,which was close to the blank group.3.Comparison of serum ALT and AST content:the LPS group was significantly higher in the LPS group than in the blank group,with significant statistical significance.Compared to the LPS group,the amount of ALT and AST in each dose group was significantly reduced.4.Comparison of serum BUN and CREA content:the amount of BUN and CREA in the LPS group was significantly increased compared to the blank group.Compared to the LPS group,the group and the treatment of each dose group were significantly reduced in serum and CREA levels.5.The ratio of TNF-α and IL-β levels in the LPS group of mice was higher than in the blank group.Compared to the LPS group,the Dex group was significantly lower in the serum of the mice in the serum of the group of mice,and the highest levels of the group were treated with low and high doses.6.Changes in the pathological section of the kidney tissue:the renal tissue of the blank group was normal;The LPS group of mice had enlarged renal tubules,and the epithelial cells were swollen and deformed,with edema,bleeding and spread of the lymphocyte.Dex group of mice treated with distal convoluted tubule epithelial cells,renal interstitial mild swelling,seepage macrophages and protein,cell infiltration,renal tubular lumen occasional loss of necrotic tissue.7.The kidney tissues NF-κB protein expression level of the signal channel:compared with the blank group,LPS group in the kidney tissues of mice NF-κB p65 and p-IκB a predominate protein expression level increased significantly;Compared to the LPS group,the group of Dex and the NF-κB p65 and p-IκB a protein in the drug group were both reduced to low,high dose groups.Conclusion1.Ginseng saponin Rb-1 can improve the SIRS model mice kidney pathological state,regulating serum ALT,AST,BUN and CREA,TNF-α and IL-1β level,it has a good therapeutic effect on SIRS renal injury.2.Ginseng saponin Rb-1 SIRS induced mice kidney injury mechanism and restraining the NF-κB cut transcriptional regulation of signal transduction pathways of inflammation factor expression,and inhibit the release of proinflammatory factor.